Morphological and Biochemical Studies of Induced and Pathological Lipidosis in Cultured Muscle Cells

  • Saïda Youcef-Khodja
  • Shimon Gatt
  • Marion Paturneau-Jouas
Part of the NATO ASI Series book series (NSSA, volume 116)


Muscle biopsies can be dissociated and cells grown as myoblasts in culture in the presence of fetal calf serum-containing medium. Myoblasts grow as single mononucleated cells which fuse to multinucleated myotubes upon replacement of fetal calf serum by horse serum in the culture medium.


Duchenne Dystrophy Horse Serum Dodecanoic Acid Culture Muscle Cell Fluorescent Derivative 
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  1. 1.
    S. Di Mauro, C. Trevisan and A. Hays, Disorders of lipid metabolism in muscle, Muscle and Nerve, 3: 369–388 (1980).PubMedCrossRefGoogle Scholar
  2. 2.
    R. Yasin, G. Van Beers, K.C. Nurse, S. Al-Ani, D.N. London and E.J. Thomson, A quantitative technique for growing human adult skeletal muscle in culture starting from mononucleated cells, J Neurol Sci, 32: 347–360 (1977).Google Scholar
  3. 3.
    C. Delaporte, M. Dehaupas and M. Fardeau, Comparison between the growth pattern of cell cultures from normal and Duchenne dystrophy muscle, J Neurol Sci, 64: 149–160 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    O. Morand, E. Fibach, N. Livni and S. Gatt, Induction of lipid storage in cultured leukemic myeloid cells by pyrene-dodecanoic acid, Biochim Biophys Acta, 793: 95–104 (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    J. Folch, M. Lees and G.H. Sloane-Stanley, A simple method for the isolation and purification of total lipids from animal tissues, J Biol Chem, 226: 497–509 (1957)PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Saïda Youcef-Khodja
    • 1
  • Shimon Gatt
    • 2
  • Marion Paturneau-Jouas
    • 2
  1. 1.INSERM U 153 Biologie et Pathologie NeuromusculairesParisFrance
  2. 2.INSERM U 134 Laboratoire de NeurochimieHôpital de la SalpêtrièreParis cedex 13France

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