Effect of Ischemia on Activities of Polyphosphoinositide Phosphodiesterase and Diacylglycerol Kinase in Gerbil Brain Synaptosomes
Ischemic insult to the CNS alters the composition and function of membranes and induces metabolic disturbances of the nerve endings (Domanska-Janik et al., 1985). A consistently observed membrane change associated with cerebral ischemia is the accumulation of free fatty acids (FFA) and a transient increase in diglyceride (DG) concentration (De Medio et al., 1980). This pool of DG formed during ischemia is subsequently degraded and can contribute significantly to the early increase in stearic and arachidonic acid (Aveldano and Bazan, 1975; DeMedio et al., 1980). DG can arise through phospholipase C action on inositol phospholipids. The breakdown of polyphosphoinositides (PPI) by a phosphodiesterase is commonly observed after the interaction of biologically active substances with their receptors (Nishizuka, 1984) and during ischemia (Strosznajder et al., 1985).
KeywordsNerve Ending Phosphatidic Acid Phosphatidic Acid Ischemic Insult Glycerol Kinase
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