Structure and Regulation of the SN-Glycerol-3-Phosphate Acyltransferase of E. Coli
The cytoplasmic membrane bound acyl-Coenzyme A:sn-glycerol-3-phosphate(glycerol-P) O-acyltransferase(EC 126.96.36.199) of Escherichia coli catalyzes the initial committed step of membrane phospholipid biosynthesis. Phospholipid biosynthesis is likely regulated, in part, by this enzyme. Studies on glycerol-P acyltransferase were limited, until recently, to crude membrane preparations or partially purified preparations. Using a combined approach of molecular genetics and biochemistry, we have attempted to bring the study of the glycerol-P acyltransferase to a molecular level and establish a suitable system for in-depth functional and regulatory studies.
KeywordsCyanogen Bromide Phospholipid Vesicle Acyltransferase Activity Phospholipid Biosynthesis Section Electron Microscopy
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