Synthesis and Properties of Fluorescent Analogs of Cytidine Diphosphate-Diacylglycerol and Phosphatidylinositol
To examine the synthesis and intracellular transport of lipids, our laboratory has developed an approach using fluorescent lipid derivatives that appear to behave as analogs of their natural counterparts. With this methodology we can examine the movements of fluorescent lipid molecules in living cells by high-resolution fluorescence microscopy, and correlate these data with the results of classical biochemical investigations (for a review see ref. 1).
KeywordsMembrane Fraction Phosphatidic Acid Cell Membrane Fraction Sulfonyl Chloride Unilamellar Liposome
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