Abstract
To examine the synthesis and intracellular transport of lipids, our laboratory has developed an approach using fluorescent lipid derivatives that appear to behave as analogs of their natural counterparts. With this methodology we can examine the movements of fluorescent lipid molecules in living cells by high-resolution fluorescence microscopy, and correlate these data with the results of classical biochemical investigations (for a review see ref. 1).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
R. E. Pagano and R. G. Sleight, Defining lipid transport pathways in animal cells, Science 229: 1051 (1985).
P. W. Majerus, E. J. Neufeld, and D. B. Wilson, Production of phosphoinositide-derived messengers, Cell 37: 701 (1984).
J. L. Marx, The polyphosphoinositides revisited, Science 224: 271 (1984).
Y. Nishizuka, The role of protein kinase C in cell surface signal transduction and tumour promotion, Nature 308: 693 (1984).
R. H. Michell, Inositol phospholipids in membrane function, Trends Biochem. Sci. 4: 128 (1979).
R. E. Pagano, K. J. Longmuir, O. C. Martin, and D. K. Struck, Metabolism and intracellular localization of a fluorescently labeled intermediate in lipid biosynthesis within cultured fibroblasts, J. Cell Biol. 91: 872 (1981).
D. K. Ford and G. Yerganian, Observations on the chromosomes of Chinese hamster cells in tissue culture, J. Natl. Cancer Inst. 21: 393 (1958)
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193: 265 (1951).
B. W. Agranoff and W. D. Suomi, Cytidine diphosphate-DL-dipalmitin, Biochem. Prep. 10: 47 (1963).
G. M. Carman and A. S. Fischl, Modification of the Agranoff-Suomi method for the synthesis of CDP-diacylglycerol, J. Food Biochem. 4: 53 (1980).
E. J. Bligh and W. J. Dyer, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol. 37: 911 (1959).
M. Hokin-Neaverson and G. S. Parries, Phosphatidylinositol synthase in mammalian pancreas in: “Inositol and phosphoinositides: metabolism and regulation” J. E. Bleasdale, J. Eichberg, and G. Hauser, eds., Humana Press, Clifton NJ (1985).
D. K. Struck, D. Hoekstra, and R. E. Pagano, Use of resonance energy transfer to monitor liposome fusion, Biochemistry 20: 4093 (1981).
B. Rouser, A. Siakotos, and S. Fleischer, Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots, Lipids 1: 85 (1966).
J. M. H. Kremer, M. W. J. v. d. Esker, C. Pathmamanoharan, and P. H. Wiersema, Vesicles of variable diameter prepared by a modified injection method, Biochemistry 17: 3932 (1977).
J. Phillipot, S. Mutaftshiev, and J. P. Liautard, A very mild method for allowing the encapsulation of very high amounts of macromolecules into very large (1000 nm) unilamellar liposomes, Biochim. Biophys. Acta 734: 143 (1983).
R. G. Sleight and R. E. Pagano, Transport of a fluorescent phosphatidylcholine analog from the plasma membrane to the Golgi apparatus, J. Cell Biol. 99: 742 (1984).
R. G. Sleight and R. E. Pagano, Transbilayer movement of a fluorescent phosphatidylethanolamine analogue across the plasma membranes of cultured mammalian cells, J. Biol. Chem. 260: 1146 (1985).
A. M. Gilfillan, A. J. Chu, D. A. Smart, and S. A. Rooney, Single plate separation of lung phospholipids including disaturated phosphatidylcholine, J. Lipid Res. 24: 1651 (1983).
W. Thompson and G. MacDonald, Isolation and characterization of cytidine diphosphate diglyceride from beef liver, J. Biol. Chem. 250: 6779 (1975).
M. Hokin-Neaverson, K. Sadeghian, D. W. Harris, and J. S. Merrin, Synthesis of CDP-diglyceride from phosphatidylinositol and CMP, Biochem. Biophys. Res. Commun. 78: 364 (1977).
T. Takenawa and K. Egawa, CDP-diglyceride:inositol transferase from rat liver. Purification and properties, J. Biol. Chem. 252: 5419 (1977).
R. H. Rao and K. P. Strickland, On the solubility, stability and partial purification of CDP-diacyl-sn-glycerol: inositol transferase from rat brain, Biochim. Biophys. Acta 348: 306 (1974).
C. Prottey and J. N. Hawthorne, The biosynthesis of phosphatidic acid and phosphatidylinositol in mammalian pancreas, Biochem. J. 105: 379 (1967).
R. E. Pagano and K. J. Longmuir, Phosphorylation, transbilayer movement, and facilitated intracellular transport of diacylglycerol are involved in the uptake of a fluorescent analog of phosphatidic acid by cultured fibroblasts, J. Biol. Chem. 260: 1909 (1985).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Uster, P.S., Pagano, R.E. (1986). Synthesis and Properties of Fluorescent Analogs of Cytidine Diphosphate-Diacylglycerol and Phosphatidylinositol. In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_64
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5212-9_64
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5214-3
Online ISBN: 978-1-4684-5212-9
eBook Packages: Springer Book Archive