Transport and Metabolism of Fatty Acids in Cultured Heart Muscle Cells from Neonatal Rats

  • Ruth Brandes
  • Arié Pinson
  • Michael Heller
Part of the NATO ASI Series book series (NSSA, volume 116)


Cultured heart muscle cells from neonatal rats take up and metabolize palmitate bound to albumin at rates higher than those for transporting fatty acids into the cells. Most of the metabolized fatty acids are esterified. The non-metabolized fatty acids comprise less than 10% of the fatty acids taken up. 2 mM of Pent-4-enoate reduced rates of palmitate conversion to CO2. “Starved” cells, devoid of glucose, in the presence of the uncoupler DNP, utilize only 20% of the supplied fatty acids but do not accumulate free fatty acids. When synthesized de novo from acetate, non-esterified fatty acids comprise 1–3% of the total fatty acids inside the myocytes and 2–13% in the extracellular medium. Diglycerides are the other export products. More than 80% of the esterified fatty acids remain inside the cells.

Decreased rates of intracellular utilization of fatty acids did not cause accumulation of fatty acids even when synthesized de novo. A putative sarcolemmal carrier may become saturated at low concentrations at the external binding site and blocked by bound FFA, preventing influx of more FFA across the sarcolemma. Intracellular accumulation of triglycerides is also prevented in myocytes due to lipolysis and export of the products — diglycerides and FFA.


Ehrlich Ascites Tumor Cell Culture Heart Cell Intracellular Lipase Esterify Fatty Acid Deplete Medium 


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  1. 1.
    Neeley, J.R. and Morgan, H.E., Relationship between carbohydrate and lipid metabolism and the energy balance of heart muscle. Ann.Rev.Physiol. 36: 413 (1974).CrossRefGoogle Scholar
  2. 2.
    Samuel, D., Paris, S. and Ailhaud, G., Uptake and metabolism of fatty acids and analogues by cultured cardiac cells from chick embryo. Eur. J.Biochem. 64: 583 (1976).PubMedCrossRefGoogle Scholar
  3. 3.
    Frelin, C., Pinson, A., Athias, P., Surville, J.M. and Padieu, P., Biochemical and electrophysiological effects of fatty acids in cultured heart cells. Pathol.Biol. 27: 45 (1979).PubMedGoogle Scholar
  4. 4.
    Spector, A.A., Steinberg, D. and Tanaka, A., Uptake of fatty acids by Ehrlich ascites tumor cells. J.Biol.Chem. 240: 1032 (1965).PubMedGoogle Scholar
  5. 5.
    Paris, S., Samuel, D., Jacques, Y., Gache, G., Francini, A. and Ailhaud, G., The role of serum albumin in the uptake of fatty acids by cultured cardiac cells from chick embryo. Eur.J.Biochem. 83: 235 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    Paris, S., Samuel, D., Romey, G. and Ailhaud, G., Uptake of fatty acids by cultured cardiac cells from chick embryo: Evidence for facilitated process without energy dependence. Biochimie 61: 361 (1979).PubMedCrossRefGoogle Scholar
  7. 7.
    Klein, K., Steinberg, R., Fiethe, B. and Overath, P., Fatty acid degradation in E. coli. Eur.J.Biochem. 19: 442 (1971).CrossRefGoogle Scholar
  8. 8.
    DeGrella, R.F. and Light, R.J., Uptake and metabolism of fatty acids by dispersed adult rat heart myocytes. II. J.Biol.Chem. 255: 9739 (1980).PubMedGoogle Scholar
  9. 9.
    Brandes, R., Arad, R. and Bar-Tana, J., Translocation of long chain fatty acids into lecithin liposomes containing the long chain fatty acyl CoA synthetase. FEBS Lett. 123: 295 (1981).PubMedCrossRefGoogle Scholar
  10. 10.
    Yagev, S., Heller, M. and Pinson, A., Changes in cytoplasmic and lysosomal enzyme activities in cultured heart cells. In Vitro 20:893 (1985).CrossRefGoogle Scholar
  11. 11.
    Rodbell, M., Metabolism of isolated fat cells. I. J.Biol.Chem. 239: 375 (1964).Google Scholar
  12. 12.
    Folch, J., Less, M. and Sloane-Stanley, G.H., A simple method for the isolation and purification of total lipids from animal tissues. J.Biol. Chem. 226: 497 (1957).Google Scholar
  13. 13.
    Kates, M., Techniques of Lipidology in Laboratory techniques in Biochemistry and molecular biology, eds. T.S.Work and E.Work, North Holland, Amsterdam, 1972, vol. 3 II, pp. 267–401.Google Scholar
  14. 14.
    Pinson, A., Desgrés, J. and Heller, M., Partial and incomplete oxidation of palmitate by cultured beating cardiac cells from neonatal rats. J.Biol.Chem. 254: 8331 (1979).PubMedGoogle Scholar
  15. 15.
    Holland, P.C. and Sherratt, H.S.A., Biochemical effects of the hypoglycemic compound Pent-4-enoic acid and related non-hypoglycemic fatty acids. Biochem.J. 136: 157 (1973).PubMedGoogle Scholar
  16. 16.
    Saggerson, E.A. and Carpenter, C.A., Carnitine palmitoyl transferase and carnitine octanoyl transferase activities in liver, kidney cortex, adipocytes, lactating mammary gland, skeletal muscle and heart. FEBS Lett. 129: 229 (1981).PubMedCrossRefGoogle Scholar
  17. 17.
    Schroedel, N.A. and Hartzell, C.R., Preferential distribution of nonesterified fatty acids to phosphatidylcholine in the neonatal mammalian myocardium. Biochem.J. 224: 651 (1984).Google Scholar
  18. 18.
    Cram, J.F., Wenger, J.I. and Neeley, J.R., Regulation of long chain fatty acid activation in heart muscle. J.Biol.Chem. 250: 73 (1975).Google Scholar
  19. 19.
    Katase, H. and Chino, H., Transport of hydrocarbons by lipophorin of insect hemolymph. Biochim.Biophys.Acta 710: 341 (1982).CrossRefGoogle Scholar
  20. 20.
    Mersel, M., Heller, M. and Pinson, A., Intracellular lipase activities in heart and skeletal muscle homogenates. Biochim.Biophys.Acta 572: 218 (1979).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Ruth Brandes
    • 1
  • Arié Pinson
    • 1
  • Michael Heller
    • 1
  1. 1.Institute of BiochemistryHebrew University - Hadassah Medical SchoolJerusalemIsrael

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