Transport of Fluorescent Fatty Acids into Cultured Cells: Analysis by the Fluorescence Activated Cell Sorter

  • Nabeel Nahas
  • Eitan Fibach
  • Haim Giloh
  • Shimon Gatt
Part of the NATO ASI Series book series (NSSA, volume 116)


The Fluorescence Activated Cell Sorter was employed for following transport of fluorescent fatty acids into cells. The time dependent uptake of 12-(1-pyrene) dodecanoic acid (P-12) by human promyelocytic leukemia (HL-60) cells and murine erythroleukemia (Friend) cells exhibited a biphasic pattern, was stimulated by glucose and markedly affected by temperature and serum albumin. Considerably less P-12 associated with Friend cells relative to HL-60 cells. Induction of erythroid differentiation resulted in a further decrease of P-12 uptake by Friend cells. These differences in the uptake of P-12 permitted identification and subsequent separation from a mixed population of cells of various lineages (HL-60 and Friend cells) as well as of different stages of maturation (uninduced and induced Friend cells).


Fluorescence Activate Cell Sorter Dodecanoic Acid Erythroid Differentiation Fluorescence Activate Cell Sorter Analysis Human Leukemic Myeloid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



12-(1-pyrene) dodecanoic acid;


Fluorescence Activated Cell Sorter


murine erythroleukemia cells;


murine erythroleukemia cells;


fetal calf serum;


bovine serum albumin;


phosphate buffered saline;




hexamethylene bisacetamide.


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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Nabeel Nahas
    • 1
  • Eitan Fibach
    • 2
  • Haim Giloh
    • 3
  • Shimon Gatt
    • 1
  1. 1.Depts. of Membrane Biochemistry and NeurochemistryThe Hebrew University Hadassah Medical SchoolJerusalemIsrael
  2. 2.Depts. of HematologyThe Hebrew University Hadassah Medical SchoolJerusalemIsrael
  3. 3.The Interdepartmental Equipment UnitThe Hebrew University Hadassah Medical SchoolJerusalemIsrael

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