Heterogeneity of Human Sphingomyelinase: Relatedness of the Major Polypeptides
Sphingomyelinase has been purified from the placenta, brain and urine (1–4). In most instances multiple polypeptides were seen. Jones et al (1) obtained a highly purified preparation from placenta which showed a major component at 89 kDa and minor ones at 70, 48 and 30 kDa, respectively. Sakuragawa found a major polypeptide for the placental enzyme at 70 kDa with a minor one at 40 kDa (2) while Yamanaka and Suzuki (3) obtained a single polypeptide at 70 kDa for the brain enzyme. Similarly Weitz et al (4) obtained bands at 73 and 28 kDa respectively for the enzyme from urine. All but the latter preparations were enriched at least 10,000-fold.
KeywordsGauche Disease Marker Lane Acid Sphingomyelinase High Molecular Weight Component Major Polypeptide
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