UDP-Glucose Sterol ß-Glucosyl Transferase, a Plant Sterol Conjugating Enzyme

  • Pierrette Bouvier-Nave
  • Pascaline Ullmann
  • Pierre Benveniste
Part of the NATO ASI Series book series (NSSA, volume 116)


Steryl glucosides and acylated steryl glucosides are present with free and esterified sterols in all plant tissues investigated so far, most probably as components of membrane structures1. The glucosylation of sterols is catalyzed by UDP-glucose sterol ß-D-glucosyl transferase (UDPG-SGTase), a plasma membrane bound enzyme of plant cells2. The reaction consists of a glucose transfer from UDP-glucose to a phytosterol with formation of a ß-glucosidic linkage between the anomeric carbon of glucose and the 3-hydroxyl group of the sterol1:
$$Sterol\,+\,UDP-glucose\,\to \,steryl\,glucoside\,+\,UDP$$
Studies in other laboratories1 and ours3 indicate clearly that UDPG-SGTase is specific for UDP-glucose and natural 4-demethyl sterols.


Anomeric Carbon Steryl Glucoside Maize Coleoptile Lipid Dependence Glucose Transfer 



uridine diphosphoglucose


UDP-glucose sterol ß-D-glucosyl transferase


uridine diphosphatase


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  1. 1.
    Z. A. Wojciechowski, The biosynthesis of plant steryl glycosides and saponins, Biochem. Soc. Trans. 11: 565 (1983).PubMedGoogle Scholar
  2. 2.
    M. A. Hartmann-Bouillon and P. Benveniste, Sterol biosynthetic capability of purified membrane fractions from maize coleoptiles, Phytochemistry 17: 1037 (1978).CrossRefGoogle Scholar
  3. 3.
    P. Ullmann, D. Rimmele, P. Benveniste and P. Bouvier-Navé, Phospholipid-dependence of plant UDP-glucose-sterol-ß-Dglucosyltransferase. H. Acetone-mediated delipidation and kinetic studies Plant Sci. Lett. 36: 29 (1984).CrossRefGoogle Scholar
  4. 4.
    R. A. Demel and B. de Kruyff, The function of sterols in membranes, Biochim. Biophys. Acta 457: 109 (1976).PubMedCrossRefGoogle Scholar
  5. 5.
    E. Quantin, M.A. Hartmann-Bouillon, F. Schuber and P. Benveniste Latency of UDPG-SGTase, a plasma membrane-bound enzyme of etiolated coleoptiles, Plant Sci. Lett. 17: 193 (1980).CrossRefGoogle Scholar
  6. 6.
    E. Quantin-Martenot, P. Benveniste, M.A. Hartmann and P. Bouvier-Navé, Activation of etiolated maize coleoptiles plasma membrane-bound UDPG-SGTase by Triton X-100, hydroxyl ions and phospholipase A2, Plant Sci. Lett. 29: 305 (1983).CrossRefGoogle Scholar
  7. 7.
    M. A. Hartmann, A. Ehrhardt and P. Benveniste, Concanavalin A binding as a tool in determining the sidedness of membrane vesicles from maize coleoptiles, Plant Sci. Lett. 30: 227 (1983).CrossRefGoogle Scholar
  8. 8.
    P. Bouvier-Navé, P. Ullmann, D. Rimmele and P. Benveniste, Phospholipid-dependence of plant UDPG-SGTase. I. Detergent-mediated delipidation by selective solubilization, Plant Sci. Lett. 36: 19 (1984).CrossRefGoogle Scholar
  9. 9.
    M. M’Voula-Tsieri, M.A. Hartmann-Bouillon and P. Benveniste, Properties of nucleosides diphosphatases in purified membrane fractions from maize coleoptiles. I. Study of latency, Plant Sci. Lett. 20: 379 (1981).CrossRefGoogle Scholar
  10. 10.
    I. H.Segel, “Enzyme Kinetics”, Wiley Interscience, New-York (1975).Google Scholar
  11. 11.
    W. W. Cleland, Statistical analysis of enzyme kinetic data, in “Methods in Enzymol.” Vol 63 p. 103 (1979).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Pierrette Bouvier-Nave
    • 1
  • Pascaline Ullmann
    • 1
  • Pierre Benveniste
    • 1
  1. 1.Laboratoire de Biochimie VégétaleE.R.A. au C.N.R.S. no 487 Institut De BotaniqueStrasbourgFrance

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