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New Techniques in Glycosyltransferase Research

  • Manju Basu
  • Kamal K. Das
  • Hung-Che Chon
  • Subhash Basu
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

Glycosyltransferases (GLTs) of eukaryotic origin catalyze the transfer of glycose units to the appropriate glycoprotein, ceramide, or glycolipid core structures. These enzymes may recognize a specific terminal glycose unit or the penultimate sugar, in addition to the terminal sugar unit.1–4 GLTs are believed to be localized in the Golgi bodies,5–7 and most of them are membrane-bound. Many have been solubilized using various detergents, and their purification is under way.8–12 We are involved in the purification of glycolipid:glycosyltransferases (GSL:GLTs) and in the study of their kinetic properties.8–13 This article describes relatively easy methods for isolation14,15 of glycolipid substrates for GSL:GLTs and the development of some convenient, relatively inexpensive assay methods.16 Both advances were essential before substantial numbers of fractions obtained during column chromatography could be assayed.

Keywords

Embryonic Chicken Sodium Metabisulfite Rabbit Bone Marrow Palmitoyl Chloride Buffy Coat Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Manju Basu
    • 1
  • Kamal K. Das
    • 1
  • Hung-Che Chon
    • 1
  • Subhash Basu
    • 1
  1. 1.Department of Chemistry Biochemistry, Biophysics and Molecular Biology ProgramUniversity of Notre DameNotre DameUSA

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