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Biosynthesis and Pharmacology of PAF-Acether (Platelet-Activating Factor)

  • Ewa Ninio
  • Danièle Nunez
  • Marc Benhamou
  • Ruth Korth
  • Francine Joly
  • Jacques Benveniste
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

Paf-acether is formed by various organs like heart or kidney and proinflammatory cells such as neutrophils, monocytes, macrophages and platelets (reviewed 1,2). This mediator is synthesized in a two-step process : 1) deacylation of 1-0-alkyl-2-acyl-sn-glycero-3-phosphocholine by a phospholipase A2 activity yielding lyso paf-acether (1-0-alkyl-sn-glycero-3-phosphocholine),3,4 2) subsequent acetylation of the latter compound by an acetyltransferase (EC 2.3.1.67).5,6 Agents interfering with phospholipase A2 activity (EDTA, mepacrine, bromophenacyl bromide) inhibit paf-acether formation from various cell types.4,7 The acetyltransferase plays the key role in paf-acether biosynthesis in the majority of cells. Several fold activation of acetyltransferase has been reported in cells stimulated with specific secretagogues.8–12

Keywords

Acetyltransferase Activity Washed Platelet Rabbit Platelet Mouse Mast Cell Bromophenacyl Bromide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Ewa Ninio
    • 1
  • Danièle Nunez
    • 1
  • Marc Benhamou
    • 1
  • Ruth Korth
    • 1
  • Francine Joly
    • 1
  • Jacques Benveniste
    • 1
  1. 1.INSERM U.200Université Paris-SudClamartFrance

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