Preliminary Study for High Performance Purification of a Hydrophobic Protein: A Biological Fluid Acetylhydrolase

  • Eric Coulais
  • Marie-Claude Prévost
  • Clotilde Cariven
  • Hugues Chap
  • Louis Douste-Blazy
Part of the NATO ASI Series book series (NSSA, volume 116)


We have previously demonstrated1 in lung alveolar washing of 6 week-old Wistar rats the presence of an acetylhydrolase activity. Because of the importance of this enzyme in different pathologies such as asthma2 where PAF-acether (platelet activating factor) is involved, we tried to have some information on its origin. Is it plasmatic exudate? Such an enzyme was described by Blank et al.3 in hypertensive rat plasma. Moreover Cox et al.4 have shown the existence of a phosphatide 2-acylhydrolase in human serum, this protein was detected by ultracentrifugal flotation in the high molecular weight lipoprotein fraction.


High Density Lipoprotein Hydrophobic Interaction Chromatography Hydrophobic Protein Hydrophobic Moiety Plasmatic Exudate 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Eric Coulais
    • 1
  • Marie-Claude Prévost
    • 1
  • Clotilde Cariven
    • 1
  • Hugues Chap
    • 1
  • Louis Douste-Blazy
    • 1
  1. 1.Biochimie des Lipides, Hôpital PurpanINSERM Unité 101ToulouseFrance

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