Preliminary Study for High Performance Purification of a Hydrophobic Protein: A Biological Fluid Acetylhydrolase
We have previously demonstrated1 in lung alveolar washing of 6 week-old Wistar rats the presence of an acetylhydrolase activity. Because of the importance of this enzyme in different pathologies such as asthma2 where PAF-acether (platelet activating factor) is involved, we tried to have some information on its origin. Is it plasmatic exudate? Such an enzyme was described by Blank et al.3 in hypertensive rat plasma. Moreover Cox et al.4 have shown the existence of a phosphatide 2-acylhydrolase in human serum, this protein was detected by ultracentrifugal flotation in the high molecular weight lipoprotein fraction.
KeywordsHigh Density Lipoprotein Hydrophobic Interaction Chromatography Hydrophobic Protein Hydrophobic Moiety Plasmatic Exudate
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- 1.M.C. Prevost, C. Cariven, M.F. Simon, H. Chap and L. Douste-Blazy. Biochem. Biophys. Res. Commun. 119: 58 (1984).Google Scholar
- 4.C.P. Cox, M.L. Wardlow, K.E. Meng, R.G. Debra and R.S. Farr, in: Symposium INSERM no 23, pp. 299–304 (1983).Google Scholar
- 5.B. Perret, Thèse d’Etat n° 1184, Université Paul Sabatier, Toulouse, (1984).Google Scholar
- 9.BioRad Technical Bulletin n° 1153.Google Scholar
- 13.E. Pfannkoch, K.C. Lu, F.E. Regnier and H.G. Barth, J. Chromatogr. Sci. 18: 430 (1980).Google Scholar