Characterization of an Acetylhydrolase Isolated from Rat Alveolar Macrophages in Comparison with the Enzyme Present in Vivo in Lung Alveoli
We have previously demonstratedl, with a 9‰ NaC1 + 5 mM EDTA lung alveolar lavage (LAL), the absence of PAF-acether in control rats. PAFacether appeared after hypoxia in lung alveoli (1.05 ± 0.5 × 10-2 nmol); under these conditions, the amount of PAF-acether still remained 1000 times lower than that of lyso-PAF-acether (12.1 ± 4.1 nmol). This could indicate either a direct liberation of the PAF-acether precursor (lyso-PAF-acether) or a secondary hydrolysis of PAF-acether by an acetylhydrolase, which might thus exert a protective action against the effects of PAF-acether.
KeywordsAlveolar Macrophage Platelet Activate Factor Lung Alveolus Alveolar Macro Secondary Hydrolysis
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