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Purification of Platelet-Activating Factor Acetylhydrolase

  • Thomas M. McIntyre
  • Diana M. Stafforini
  • Guy A. Zimmerman
  • Stephen M. Prescott
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

The only known phospholipid with potent biologic activity, Platelet-activating Factor (PAF, PAF-Acether), has been identified as 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (1-alkyl-2-acetyl-GPC).1 Among the diverse effects of PAF are the activation of platelets, neutrophils and monocytes, and the induction of thrombocytopenia, neutropenia, hypotension and vasopermeability in whole animals. The biologic activity of PAF depends upon the presence of a short chain acyl group at the sn-2 position, and hydrolysis of this ester destroys bioactivity. This reaction is catalyzed by a plasma component that was originally described as “acid-labile factor”2, but now has been identified as PAF-acetylhydrolase. We find that this activity is responsible for nearly all of the initial metabolism of PAF in whole blood, and have partially purified and characterized this activity.

Keywords

Critical Micellar Concentration Lipoprotein Particle Potent Biologic Activity High Molecular Weight Aggregate Lipid Interface 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    F. Snyder, Chemical and biochemical aspects of platelet-activating factor: A novel class of ether-linked choline phospholipids, Medicinal Res. Rev. 5: 107 (1985).CrossRefGoogle Scholar
  2. 2.
    R.N. Pickard, R. Farr and D.J. Hanahan, Physiochemical and functional identity of rabbit platelet-activating factor (PAF) released in vivo during IgE anaphylaxis with PAF released in vitro from IgE-sensitized basophils, J. Immunol. 123: 1847 (1979).Google Scholar
  3. 3.
    M.L. Blank, Tc. Lee, V. Fitzgerald and F. Snyder, A specific acetylhydrolase for 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (a hypotensive and platelet-activating lipid), J. Biol. Chem. 256: 175 (1981).PubMedGoogle Scholar
  4. 4.
    I. Alam, J.B. Smith and M.J. Silver, Metabolism of platelet-activating factor by blood platelets and plasma, Lipids 18: 534 (1983).PubMedCrossRefGoogle Scholar
  5. 5.
    R.S. Farr, C.P. Cox, M.L. Wardlow and R. Jorgensen, Preliminary studies of an acid-labile factor (ALF) in human sera that inactivates platelet-activating factor (PAF), Clin. Immunol. and Immunopathol. 15: 318 (1980).CrossRefGoogle Scholar
  6. 6.
    M. Burstein, Lipoprotein-polyanion-metal interactions, Adv Lipid Res 11: 67 (1973).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Thomas M. McIntyre
    • 1
  • Diana M. Stafforini
    • 1
  • Guy A. Zimmerman
    • 1
  • Stephen M. Prescott
    • 1
  1. 1.Nora Eccles Harrison Cardiovascular Research and Training InstituteUniversity of UtahSalt Lake CityUSA

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