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Some Properties of Lysoplasmalogenase and Alkenylhydrolase from Rat Liver Microsomes

  • Ulrich Franken
  • Hildegard Debuch
  • Johannes Gunawan
  • Achim Harder
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

The existence of lysoplasmalogenase acting on ethanolaminelysoplasmalogen (1-alk-1’enyl-sn-glycero-3-phosphoethanolamine: alkenyl GPE) is now well established and is shown to occur in rat liver (Gunawan and Debuch, 1981) and rat brain (Gunawan and Debuch, 1982). We used a substrate 14C labeled within the aldehyde residue. Thus the radioactive aldehyde,liberated by the enzyme from the lyso compound was isolated as well as GPE in a 1:1 molar ratio (Gunawan and Debuch, 1985).

Keywords

Soya Bean Microsomal Enzyme Liver Microsomal Enzyme Microsomal Enzyme Activity Microsomal Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Arion,W.J., Ballas,L.M., Lange,A.J., and Wallin,B.K., 1976, Microsomal membrane permeability and the hepatic glucose 6-phosphate system: interactions of the system with D-mannose 6-phosphate and D-mannose, J. Biol. Chem. 251: 4901Google Scholar
  2. Bartlett,G.R., 1958, Phosphorous assay in column chromatography, J. Biol. Chem. 234: 466Google Scholar
  3. Bell,R.M., Ballas, L.M., and Coleman, R.A., 1981, Lipid topogenesis, J. Lipid Res. 22: 391PubMedGoogle Scholar
  4. Coleman, R., and Bell, R.M., 1978, Evidence that biosynthesis of phosphatidylethanolamine, phosphatidylcholine, and triacylglycerol occurs on the cytoplasmic side of microsomal vesicles, J. Cell Biol. 76: 245PubMedCrossRefGoogle Scholar
  5. Ellingson, J. S., and Lands, W. E. M., 1968, Phospholipid Reactivation of Plasmalogen Metabolism, Lipids, 3: 111PubMedCrossRefGoogle Scholar
  6. Gunawan,J., and Debuch,H., 1981, Liberation of free aldehyde from 1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine (lysoplasmalogen) by rat liver microsomes, Hoppe-Seyler’s Z. Physiol. Chem. 362: 445PubMedCrossRefGoogle Scholar
  7. Gunawan,J., and Debuch, H.,1982, Lysoplasmalogenase - A Microsomal Enzyme from Rat Brain, J. Neurochem. 39: 693Google Scholar
  8. Gunawan,J., and Debuch,H., 1985, Alkenylhydrolase: A Microsomal Enzyme Activity in Rat Brain, J. Neurochem. 44: 370PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Ulrich Franken
    • 1
  • Hildegard Debuch
    • 1
  • Johannes Gunawan
    • 1
  • Achim Harder
    • 1
  1. 1.Institut f. Physiol. ChemieUniversity Köln W-GermanyKöln 41Germany

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