Advertisement

Partial Characterization of the Cytosolic Phospholipase A1 of Rat Heart

  • Gilles Nalbone
  • Karl Y. Hostetler
  • Jeannie Leonardi
  • Huguette Lafont
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

In heart the maintenance of membrane integrity is essential for many cell functions including the need to control intracellular calcium concen­tration to ensure optimal inotropic conditions. In myocardial ischemia alterations in membrane phospholipids metabolism1–5 are thought to be of great importance since phospholipid degradation products, fatty acids and lysophospholipids, are potentially cytotoxic and may cause irregular heart rhythms6,7. The membrane phospholipids are subject to the action of intracel­lular phospholipases and lysophospholipases and detailed information about these enzymes are needed to allow a better understanding of membrane events in myocardial ischemia.

Keywords

Membrane Phospholipid Cytosolic Phospholipase Recovered Activity Acylase Activity Irregular Heart Rhythms6 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    K.A. Chien, J.P. Reeves, L.M. Buza, F. Bonte, R.W. Parkey and J.T. Willer-son, Phospholipid alterations in canine ischemic myocardium. Circ. Res. 48: 711 (1981).PubMedCrossRefGoogle Scholar
  2. 2.
    F.W. Prinzen, G.J. Van der Vusse, T.H.M. Roemen, W.A. Coumans and R.S. Reneman, Accumulation of nonesterified fatty acids in ischemic canine myocardium. Am. J. Physiol. 247: H264 (1984).PubMedGoogle Scholar
  3. 3.
    R.Y.K. Man, T.L. Slater, M.P. Pelletier and P.C. Choy, Alterations of phospholipids in ischemic canine myocardium during acute arrhythmia. Lipids 18: 677 (1983).PubMedCrossRefGoogle Scholar
  4. 4.
    C. Steenbergen and R.B. Jennings, Relationship between lysophospholipid accumulation and plasma membrane injury during total in vitro ischemia in dog heart. J. Mol. Cell Cardiol. 16: 605 (1984).PubMedCrossRefGoogle Scholar
  5. 5.
    K.R. Chien, A. Sen, R. Reynolds, A. Chang, Y. Kim, M.D. Gunn, L. Maximilian Buja and J.T. Willerson, Release of arachidonate from membrane phospholipids in cultured neonatal rat myocardial cells during adenosine triphosphate depletion. J. Clin. Invest. 75: 1770 (1985).PubMedCrossRefGoogle Scholar
  6. 6.
    R.Y.K. Man, T. Wong and P.C. Choy, Effects of lysophosphoglycerides on cardiac arrhythmias. Life Sciences 32: 1325 (1983).PubMedCrossRefGoogle Scholar
  7. 7.
    F.B. Corr and B.E. Sobel, Arrhythmogenic properties of phospholipid metabolites associated with myocardial ischemia. Federation Proc. 42: 2454 (1983).Google Scholar
  8. 8.
    J.W. Palmer, P.C. Schmid, D.R. Pfeiffer and H.H.O. Schmid, Lipids and lipolytic enzyme activities of rat heart mitochondria. Arch. Biochem. Biophys. 241: 674 (1981).CrossRefGoogle Scholar
  9. 9.
    R.F. Franson, M. Waite and W.B. Weglicki, Phospholipase A activity of lysosomes of rat myocardial tissue. Biochemistry 11: 472 (1972).PubMedCrossRefGoogle Scholar
  10. 10.
    S.W. Tam, R.Y.K. Man and P.C. Choy, The hydrolysis of phosphatidylcholine by phospholipase A2 in hamster heart. Can. J. Biochem. Cell. Biol. 62: 1269 (1984).PubMedCrossRefGoogle Scholar
  11. 11.
    W.B. Weglicki, M. Waite, P. Sisson and S.B. Shohet, Myocardial phospholipase A of microsomal and mitochondrial fractions. Biochim. Biophys. Acta 231: 512 (1971).PubMedCrossRefGoogle Scholar
  12. 12.
    G. Nalbone and K.Y. Hostetler, Subcellular localization of the phospholipases A of rat heart: evidence for a cytosolic phospholipase Al. J. Lipid Res. 26: 104 (1985).PubMedGoogle Scholar
  13. 13.
    M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72: 248 (1976).PubMedCrossRefGoogle Scholar
  14. 14.
    K.Y. Hostetler, P.J. Yasaki and H. Van den Bosch, Purification of lysosomal phospholipase A: evidence for multiple isoenzymes. J. Biol. Chem. 257: 13367 (1982).PubMedGoogle Scholar
  15. 15.
    H. Van den Bosch and A.J. Aarsman, A review on methods of phospholipase determination. Agents Actions 9: 382 (1979).PubMedCrossRefGoogle Scholar
  16. 16.
    J. Folch, M. Lees and G.H. Sloane Stanley, A simple procedure for the isolation one purification of total lipids from animal tissues. J. Biol. Chem. 226: 497 (1957).PubMedGoogle Scholar
  17. 17.
    R.T. Gross and B.E. Sobel, Rabbit myocardial cytosolic lysophospholipase. J. Biol. Chem. 258: 5221 (1983).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Gilles Nalbone
    • 1
  • Karl Y. Hostetler
    • 1
    • 2
  • Jeannie Leonardi
    • 1
  • Huguette Lafont
    • 1
  1. 1.INSERM U-130MarseilleFrance
  2. 2.University of California and Veterans Administration HospitalLa JollaUSA

Personalised recommendations