Endogenous Suppression of Neutral-Active and Calcium-Dependent Phospholipase A2 Activity in Human Polymorphonuclear Leukocytes
Neutral-active and calcium-dependent phospholipase A2 (PLA2) activity associated with cell granules and membranes has been observed previously in homogenates of human and rabbit polymorphonuclear leukocytes (PMNs)1–3. In these studies enzyme activity measured under similar assay conditions was more than ten times lower in the human than in the rabbit enzyme. We therefore investigated whether PLA2 activity of human PMN homogenates might be partly suppressed by an endogenous inhibitor.
KeywordsMethyl Oleate Elaidic Acid Soluble Extract PLA2 Activity Resuspended Pellet
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