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Some Properties of Membrane-Bound Phospholipases A2

  • A. J. Aarsman
  • H. B. M. Lenting
  • F. W. Neys
  • C. F. P. Roosenboom
  • J. M. de Winter
  • H. van den Bosch
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

Phospholipase A2 (EC 3.1.1.4) is found in high concentrations in venoms of bees, snakes and scorpions (1), and also in pancreatic juice (2). These soluble enzymes are nowadays well characterized (1,3). Much less is known about the cellular phospholipases A2, although these enzymes are thought to be involved in many important cellular processes like phospholipid turnover and generation of free arachidonic acid. This polyunsaturated fatty acid is the rate-limiting precursor in prostaglandins, leukotrienes, thromboxane and prostacyclin formation.

Keywords

Pancreatic Juice Platelet Lysate Platelet Suspension Important Cellular Process Peripheral Membrane Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    H.M. Verheij, A.J. Slotboom, and G.H. de Haas, Structure and function of phospholipase A2, Rev. Physiol. Biochem. Pharmacol. 91: 91 (1981).PubMedGoogle Scholar
  2. 2.
    C. Figarella, F. Clemente, and O. Guy, A zymogen of phospholipase A in human pancreatic juice, Biochim. Biophys. Acta 227: 213 (1971).PubMedCrossRefGoogle Scholar
  3. 3.
    A.J. Slotboom, H.M. Verheij, and G.H. de Haas, On the mechanism of phospholipase A2, in: “New Comprehensive Biochemistry”, Vol. 4, pp. 359–434, J.N. Hawthorne and G.B. Ansell, eds., Elsevier Biomedical, Amsterdam (1982).Google Scholar
  4. 4.
    H. van den Bosch, Phospholipases, in: “New Comprehensive Biochemistry”, Vol. 4, pp. 313–357, J.N. Hawthorne and G.B. Ansell, eds., Elsevier Biomedical, Amsterdam (1982).Google Scholar
  5. 5.
    A.J. Aarsman, C.F.P. Roosenboom, G.E.W. van Geffen, and H. van den Bosch, Some aspects of rat platelet and serum phospholipase A2 activities,Biochim. Biophys. Acta, accepted for publication (1985).Google Scholar
  6. 6.
    J. Etienne, A. Grüber, and J. Polonovski, Phospholipases A2 activables des plaquettes sanguines de rat, Biochimie 64: 377 (1982).PubMedCrossRefGoogle Scholar
  7. 7.
    J.M. de Winter, G.M. Vianen, and H. van den Bosch, Purification of rat liver mitochondrial phospholipase A2, Biochim. Biophys. Acta 712: 332 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    A.J. Aarsman, F. Neys, and H. van den Bosch, A simple and versatile affinity column for phospholipase A2, Biochim. Biophys. Acta 792: 363 (1984).PubMedCrossRefGoogle Scholar
  9. 9.
    R.M. Kramer, C. Wüthrich, C. Bollier, P.R. Allegrini, and P. Zahler, Isolation of phospholipase A2 from sheep erythrocyte membranes in the presence of detergents, Biochim. Biophys. Acta 507: 381 (1978).CrossRefGoogle Scholar
  10. 10.
    C.O. Rock and F. Snyder, Rapid purification of phospholipase A2 from Crotalus adamanteus venom by affinity chromatography, J. Biol. Chem. 250: 6564 (1975).PubMedGoogle Scholar
  11. 11.
    R.E. Barden, P.L. Darke, R.A. Deems, and E.A. Dennis, Interaction of phospholipase A2 from cobra venom with Cihacron Blue F3GA, Biochemistry 19: 1621 (1980).PubMedCrossRefGoogle Scholar
  12. 12.
    J. Etienne, A. Grüber, and J. Polonovski, Activité phospholipasique A2 du sérum de rat. Association de deux protéines, Biochim. Biophys. Acta 619: 693 (1980).PubMedCrossRefGoogle Scholar
  13. 13.
    G.L. Scherphof, M. Waite, and L.L.M. van Deenen, Formation of lysophosphatidylethanolamines in cell fractions of rat liver, Biochim. Biophys. Acta 125: 406 (1966).CrossRefGoogle Scholar
  14. 14.
    M. Waite and P. Sisson, Partial purification and characterization of the phospholipase A2 from rat liver mitochondria, Biochemistry 10: 2377 (1971).PubMedCrossRefGoogle Scholar
  15. 15.
    Y. Fujiki, A.L. Hubbard, S. Fowler, and P.B. Lazarow, Isolation of intracellular membranes by means of sodium carbonate treatment: Application to endoplasmic reticulum, J. Cell. Biol. 93: 97 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • A. J. Aarsman
    • 1
  • H. B. M. Lenting
    • 1
  • F. W. Neys
    • 1
  • C. F. P. Roosenboom
    • 1
  • J. M. de Winter
    • 1
  • H. van den Bosch
    • 1
  1. 1.Laboratory of BiochemistryState University of UtrechtUtrechtThe Netherlands

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