Comparison of the Activation of Soluble and Immobilized Phospholipase A2
Phospholipase A2 is perhaps the most well-studied and well-characterized enzyme of phospholipid breakdown and as such serves as a paradigm or model system for the study of enzymes of lipid metabolism (Review: 1). Over the years, our laboratory has used a variety of approaches to study the phospholipase A2 from the venom of the Indian cobra (Naja naja naja) as summarized elsewere (2). Among these approaches, we (3) prepared an immobilized form of the enzyme and studied its kinetic properties; this study was published in 1978.
KeywordsImmobilize Enzyme Soluble Enzyme Immobilize Form Cobra Venom Naja Naja
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- 7.Roberts, M.F., Deems, R.A., and Dennis, E.A., Proc Natl. Acad. Sci. U.S.A. 74, 1950–1954.Google Scholar
- 9.Plückthun, A., and Dennis, E.A. J. Biol. Chem. 260, 11099–11106.Google Scholar
- 13.Lombardo, D., and Dennis, E.A. (1985) Fed. Proc. 44, 1204.Google Scholar
- 14.Lombardo, D., and Dennis, E.A. (1985) J. Biol. Chem, 260., in press.Google Scholar