Advertisement

Comparison of the Activation of Soluble and Immobilized Phospholipase A2

  • Dominique Lombardo
  • Edward A. Dennis
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

Phospholipase A2 is perhaps the most well-studied and well-characterized enzyme of phospholipid breakdown and as such serves as a paradigm or model system for the study of enzymes of lipid metabolism (Review: 1). Over the years, our laboratory has used a variety of approaches to study the phospholipase A2 from the venom of the Indian cobra (Naja naja naja) as summarized elsewere (2). Among these approaches, we (3) prepared an immobilized form of the enzyme and studied its kinetic properties; this study was published in 1978.

Keywords

Immobilize Enzyme Soluble Enzyme Immobilize Form Cobra Venom Naja Naja 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dennis, E.A. (1983) The Enzymes, XVI, pp. 307–353.CrossRefGoogle Scholar
  2. 2.
    Dennis, E.A., Dar’ke, P.L., Deems, R.A., Kensil, C.R., and Pldckthun, A. (1981) Mol. Cell. Biochem. 36, 37–45.PubMedCrossRefGoogle Scholar
  3. 3.
    Adamich, M., Voss, H.F., and Dennis, E.A. (1978) Arch. Biochem. Biophys. 189, 417–423.PubMedCrossRefGoogle Scholar
  4. 4.
    Roberts, M.F., Adamich, M., Robson, R.J., and Dennis, E.A. (1979) Biochemistry 18, 3301–3308.PubMedCrossRefGoogle Scholar
  5. 5.
    Adamich, M., Roberts, M.F., and Dennis, E.A. (1979) Biochemistry 18, 3308–3313.PubMedCrossRefGoogle Scholar
  6. 6.
    Pldckthun, A., and Dennis, E.A. (1982) Biochemistry 21, 1750–1756.CrossRefGoogle Scholar
  7. 7.
    Roberts, M.F., Deems, R.A., and Dennis, E.A., Proc Natl. Acad. Sci. U.S.A. 74, 1950–1954.Google Scholar
  8. 8.
    Hazlett, T.L., and Dennis, E.A. (1982) Biochemistry 21, 1750–1756.CrossRefGoogle Scholar
  9. 9.
    Plückthun, A., and Dennis, E.A. J. Biol. Chem. 260, 11099–11106.Google Scholar
  10. 10.
    Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5734–5739.PubMedGoogle Scholar
  11. 11.
    Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5740–5744.PubMedGoogle Scholar
  12. 12.
    Pldckthun, A., Rohlfs, R., Davidson, F.F., and Dennis, E.A. (1985) Biochemistry 24, 4201–4208.CrossRefGoogle Scholar
  13. 13.
    Lombardo, D., and Dennis, E.A. (1985) Fed. Proc. 44, 1204.Google Scholar
  14. 14.
    Lombardo, D., and Dennis, E.A. (1985) J. Biol. Chem, 260., in press.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Dominique Lombardo
    • 1
  • Edward A. Dennis
    • 1
  1. 1.Department of ChemistryUniversity of California at San DiegoLa JollaUSA

Personalised recommendations