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Comparison of the Activation of Soluble and Immobilized Phospholipase A2

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Enzymes of Lipid Metabolism II

Part of the book series: NATO ASI Series ((NSSA,volume 116))

Abstract

Phospholipase A2 is perhaps the most well-studied and well-characterized enzyme of phospholipid breakdown and as such serves as a paradigm or model system for the study of enzymes of lipid metabolism (Review: 1). Over the years, our laboratory has used a variety of approaches to study the phospholipase A2 from the venom of the Indian cobra (Naja naja naja) as summarized elsewere (2). Among these approaches, we (3) prepared an immobilized form of the enzyme and studied its kinetic properties; this study was published in 1978.

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References

  1. Dennis, E.A. (1983) The Enzymes, XVI, pp. 307–353.

    Article  Google Scholar 

  2. Dennis, E.A., Dar’ke, P.L., Deems, R.A., Kensil, C.R., and Pldckthun, A. (1981) Mol. Cell. Biochem. 36, 37–45.

    Article  PubMed  CAS  Google Scholar 

  3. Adamich, M., Voss, H.F., and Dennis, E.A. (1978) Arch. Biochem. Biophys. 189, 417–423.

    Article  PubMed  CAS  Google Scholar 

  4. Roberts, M.F., Adamich, M., Robson, R.J., and Dennis, E.A. (1979) Biochemistry 18, 3301–3308.

    Article  PubMed  CAS  Google Scholar 

  5. Adamich, M., Roberts, M.F., and Dennis, E.A. (1979) Biochemistry 18, 3308–3313.

    Article  PubMed  CAS  Google Scholar 

  6. Pldckthun, A., and Dennis, E.A. (1982) Biochemistry 21, 1750–1756.

    Article  Google Scholar 

  7. Roberts, M.F., Deems, R.A., and Dennis, E.A., Proc Natl. Acad. Sci. U.S.A. 74, 1950–1954.

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  8. Hazlett, T.L., and Dennis, E.A. (1982) Biochemistry 21, 1750–1756.

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  9. Plückthun, A., and Dennis, E.A. J. Biol. Chem. 260, 11099–11106.

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  10. Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5734–5739.

    PubMed  CAS  Google Scholar 

  11. Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5740–5744.

    PubMed  CAS  Google Scholar 

  12. Pldckthun, A., Rohlfs, R., Davidson, F.F., and Dennis, E.A. (1985) Biochemistry 24, 4201–4208.

    Article  Google Scholar 

  13. Lombardo, D., and Dennis, E.A. (1985) Fed. Proc. 44, 1204.

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  14. Lombardo, D., and Dennis, E.A. (1985) J. Biol. Chem, 260., in press.

    Google Scholar 

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© 1986 Plenum Press, New York

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Lombardo, D., Dennis, E.A. (1986). Comparison of the Activation of Soluble and Immobilized Phospholipase A2 . In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_18

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  • DOI: https://doi.org/10.1007/978-1-4684-5212-9_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5214-3

  • Online ISBN: 978-1-4684-5212-9

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