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Mechanism of Interaction of Phospholipase A2 with Phospholipid Substrates and Activators

  • Edward A. Dennis
  • Andreas Plückthun
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

Phospholipase A2 is one of the smallest and simplest enzymes of lipid metabolism (1). Over the last several years, our laboratory has been focusing on the mechanism by which it interacts with phospholipid in the lipid-water interface and achieves an extraordinary increase in activity over manomerically dispersed substrate. This increase is observed on both micelles of synthetic short chain phospholipid substrates and on mixed micelles with detergents such as Triton X-100. This requirement for an interface for maximal activity has puzzled enzymologists for years.

Keywords

Immobilize Enzyme Mixed Micelle Soluble Enzyme Fatty Acid Product Cobra Venom 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Dennis, E.A. (1983) in “The Enzymes” (P.D. Boyer, ed.) Academic Press, New York, Vol. 16, pp. 307–353.Google Scholar
  2. 2.
    Roberts, M.F., Adamich, M., Robson, R.J., and Dennis, E.A. (1979) Biochemistry 18, 3301–3308.PubMedCrossRefGoogle Scholar
  3. 3.
    Adamich, M., Roberts, M.F., and Dennis, E. A. (1979) Biochemistry 18, 3308–3313.PubMedCrossRefGoogle Scholar
  4. 4.
    Plückthun, A., Rohlfs, R., Davidson, F.F., and Dennis, E.A. (1985) Biochemistry 24, 4201–4208.PubMedCrossRefGoogle Scholar
  5. 5.
    Hazlett, T.L., and Dennis, E.A. (1985) Biochemistry, 24. In press.Google Scholar
  6. 6.
    Plackthun, A., and Dennis, E.A. (1985) J. Biol. Chem. 260, 11099–11106.Google Scholar
  7. 7.
    Imnbardo, D., and Dennis, E.A. (1985) J. Biol. Chem., 260. In press.Google Scholar
  8. 8.
    Lombardo, D., and Dennis, E.A. (1985) J. Ból. Chem. 260, 7234–7240.Google Scholar
  9. 9.
    Roberts, M.F., Deems, R.A., and Dennis, E.A. (1977) Prod. Natl. Acad. Sci. USA 74, 1950–1954.CrossRefGoogle Scholar
  10. 10.
    Roberts, M.F., Otnaess, A.B., Kensil, C.R., and Dennis, E.A. (1978) J. Biol. Chem. 253, 1252–1257.PubMedGoogle Scholar
  11. 11.
    ToTtiacthun, A., and Dennis, E.A. (1982) Biochemistry 21, 1750–1756.CrossRefGoogle Scholar
  12. 12.
    Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5740–5744.PubMedGoogle Scholar
  13. 13.
    Dennis, E.A. (1973) J. Lipid Res. 14, 152–159.PubMedGoogle Scholar
  14. 14.
    Dennis, E.A. (1973) Arch. Biochem. Biophys. 158, 485–493.PubMedCrossRefGoogle Scholar
  15. 15.
    Deems, R.A., Eaton, B.R., and Dennis, E.A. (1975) J. Biol. Chem. 250, 9013–9020.PubMedGoogle Scholar
  16. 16.
    Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5734–5739.PubMedGoogle Scholar
  17. 17.
    Deems, R.A., and Dennis, E.A. (1981) Methods in Enzymology, Lipids 71, 703–710.CrossRefGoogle Scholar
  18. 18.
    Albery, J., and Knowles, J. (1977) Angew. Chemie ( Int. Ed. ) 16, 285–293.CrossRefGoogle Scholar
  19. 19.
    Ray, W.J. (1983) Biochemistry 22, 4625–4637.PubMedCrossRefGoogle Scholar
  20. 20.
    Kensil, C.R., and Dennis, E.A. (1981) Biochemistry 21, 6079–6085.CrossRefGoogle Scholar
  21. 21.
    Verheij, H.J., Slotboan, A.J., and de Haas, G.H. (1981) Rev. Physiol. Biochem. Pharmacol. 91, 91–203.PubMedGoogle Scholar
  22. 22.
    van Eijk, J.H., Verheij, H.M., Dijkman, R., and de Haas, G.H. (1983) Eur. J. Biochem. 132, 183–188.PubMedCrossRefGoogle Scholar
  23. 23.
    Halle, J.D.R., Egmond, M.R., Dikman, R., van Oort, M.G., Jirgensons, B., and de Haas, G.H. (1983) Biochemistry 22, 5347–5353.CrossRefGoogle Scholar
  24. 24.
    Hille, J.D.R., Egmond, M.R., Dijkman, R., van Oort, M.G., Sauve, P., and de Haas, G.H. (1983) Biochemistry 22, 5353–5358.PubMedCrossRefGoogle Scholar
  25. 25.
    Dennis, E.A., Darke, P.L., Deems, R.A., Kensil, C.R., and Plitckthun, A. (1981) Mol. Cell. Biochem. 36, 37–45.PubMedCrossRefGoogle Scholar
  26. 26.
    Wells, M.A. (1972) Biochemistry 11, 1030–1041.PubMedCrossRefGoogle Scholar
  27. 27.
    Keith, C., Feldman, D.S., Deganello, S., Glick, J., Ward, K.B., Jones, E.O., and Sigler, P.B. (1981) J. Biol. Chem. 256, 8602–8607.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Edward A. Dennis
    • 1
  • Andreas Plückthun
    • 1
  1. 1.Department of ChemistryUniversity of California at San DiegoLa JollaUSA

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