Treatment of Rat Brain Microsomal Vesicles with Octyl-β-D-Glucopyranoside: A Study on Ethanolamine Base-Exchange after Reaggregation
The base-exchange reaction can form phosphatidylethanolamine (PE) by displacement of a lipid-bound base with free ethanolamine (Porcellati et al., 1971). The reaction requires Ca2+ and it is usually measured incubating free radioactive ethanolamine with membranes in a suitable medium. The PE synthesized in vitro through base-exchange in rat brain microsomes cannot mix readily with the bulk of membrane PE, as shown measuring the reactivity of the lipid with trinitrobenzenesulfonic acid (TNBS) (Corazzi et al., 1983); however, the compartmentation of the newly-formed PE is lost if membranes are mixed with disgregating concentrations of various detergents (Corazzi et al., 1983).
KeywordsMembrane Component Critical Micellar Concentration Trinitrobenzenesulfonic Acid Brain Microsome Sarcoplasmic Reticulum Vesicle
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