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Molecular and Cellular Aspects of Reproduction pp 53–85Cite as

Biosynthetic Controls That Determine the Branching and Microheterogeneity of Protein-Bound Oligosaccharides

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 205))

Abstract

The past ten years have witnessed an unprecedented explosion in our knowledge of complex carbohydrate fine structure. The techniques which have been responsible for these advances include high resolution gel filtration chromatography, high performance liquid chromatography, the availability of a battery of exo- and endo-glycosidases with well-characterized specificities, new chemical methods for cleaving carbohydrate-amino acid linkages, high field nuclear magnetic resonance spectrometry, and high resolution mass spectrometry. It is clear that two properties distinguish complex carbohydrates from proteins and nucleic acids, the other two classes of biological macromolecules, namely: 1) branching and 2) microheterogeneity. These properties pose both challenges and problems for researchers interested in the function and biosynthesis of complex carbohydrates.

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Authors and Affiliations

  1. Research Institute, Hospital for Sick Children, and Department of Biochemistry, University of Toronto, Toronto, Ontario, M5G 1X8, Canada

    Harry Schachter

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  1. Harry Schachter
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Editors and Affiliations

  1. Reproductive Biology Study Section, Division of Research Grants, National Institutes of Health, Westwood Building, Room 307, 20205, Bethesda, Maryland, USA

    Dharam S. Dhindsa D.V.M., Ph.D. (Executive Secretary/Referral Officer) (Executive Secretary/Referral Officer)

  2. Department of Biological Sciences, State University of New York, Cooke Hall 347, 14260, Buffalo, New York, USA

    Om P. Bahl Ph.D. (Professor of Biological Sciences) (Professor of Biological Sciences)

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© 1986 Plenum Press, New York

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Schachter, H. (1986). Biosynthetic Controls That Determine the Branching and Microheterogeneity of Protein-Bound Oligosaccharides. In: Dhindsa, D.S., Bahl, O.P. (eds) Molecular and Cellular Aspects of Reproduction. Advances in Experimental Medicine and Biology, vol 205. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5209-9_2

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  • DOI: https://doi.org/10.1007/978-1-4684-5209-9_2

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