Mammalian Gene Transfer and Gene Expression
It has long been the desire of molecular biologists to be able to study the regulation of expression of specific mammalian genes. With the advent of recombinant DNA technology and the availability of specific cloned genes, several new approaches to these studies have emerged. One of the most promising, which we will discuss in detail here, is the introduction of specific cloned genes into the genome of embryos and the development of adult animals from these embryos which also contain these cloned DNA sequences in their genomes. This whole animal system has many advantages. The genes may be present in a very native biological environment. If the gene is stably integrated and passed from generation to generation, a large number of similar animals would be available for study. As animals of all stages of development could be obtained, it should be possible to study specific developmental changes occurring within the integrated DNA. This information could be amplified by introducing genes with specific deletions or mutations to analyze which portions of the genes are important in genetic regulation and developmental control of these systems. The opportunities for this approach to the study of the regulation of expression of cloned genes in these whole animal systems seem extensive.
KeywordsTransgenic Mouse Thymidine Kinase Transgenic Animal Insertional Mutagenesis Mouse Mammary Tumor Virus
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