Organization and Expression of Gonadotropin Genes

  • William W. Chin
  • Soheyla D. Gharib
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 205)


Lutropin (LH) and follitropin (FSH) are gonadotropins produced and secreted by the mammalian anterior pituitary gland. They serve to regulate gonadal function by stimulating the production of sex steroid hormones (estrogen, progesterone, and testosterone) and gametogenesis. The gonadotropins are the effectors of well-regulated gonadal development and function orchestrated in a finely tuned manner by factors from the hypothalamus. Hence, LH and FSH play critical roles in sexual development and maturation as well as normal reproductive function (Kalra and Kalra, 1985).


Luteinizing Hormone Chorionic Gonadotropin Subunit Gene Glycoprotein Hormone Serum Luteinizing Hormone 
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  1. Acher, R., 1980, Molecular evolution of biologically active polypeptides, Proc. R. Soc. London (Biol.) 200:21–43.CrossRefGoogle Scholar
  2. Alexander, D. C., and Miller, W. L., 1982, Regulation of ovine follicle-stimulating hormone 6 chain mRNA by 176-estradiol in vivo and in vitro, J. Biol. Chem. 257:2282–2286.PubMedGoogle Scholar
  3. Beck, A., Vellucci, V., and Curry, K., 1985, Cloning and expression of DNAs coding for follicle-stimulating hormone, DNA, 4:76.Google Scholar
  4. Boothby, M., Ruddon, R. W., Anderson, C., McWilliams, D., and Boime, I., 1982, A single gonadotropin a subunit gene in normal tissue and tumor derived cell-lines, J. Biol. Chem. 256:5121–5127.Google Scholar
  5. Carr, F. E., Need, L. R., Shin, L., and Chin, W. W., 1985, Isolation and characterization of the gene encoding the 6-subunit of rat thyrotropin, 9th Intl. Thyroid Cong. Sao Paulo, Brazil, Abstract #13.Google Scholar
  6. Carr, F. E., and Chin, W. W., 1985, Absence of detectable chorionic gonadotropin mRNAs in rat placenta during gestation, Endocrinology 116:1151–1157.PubMedCrossRefGoogle Scholar
  7. Chin, W. W., 1985, Organization and expression of glycoprotein hormones gene, in: “The Pituitary Gland,” H. Imura, ed., Raven Press, New York, pp. 103–125.Google Scholar
  8. Chin, W. W., Godine, J. E., Klein, D. R., Chang, A. S., Tan, L. K., and Habener J. F., 1983, Nucleotide sequence of the cDNA encoding the precursor of the a subunit of rat lutropin, Proc. Natl. Acad. Sci. USA, 80:4649–4653.PubMedCrossRefGoogle Scholar
  9. Corbani, M., Counis, R., Starzec, A., and Jutisz, M., 1984, Effect of gonadectomy on pituitary levels of mRNA encoding gonadotropin subunits and secretion of luteinizing hormone, Mol. Cell. Endo. 35:83–87.CrossRefGoogle Scholar
  10. Counis, R., Corbani, M., and Jutisz, M., 1983, Estradiol regulates mRNAs encoding precursors to rat lutropin (LH) and follitropin (FSH) subunits, Biochem. Biophys. Res. Comm. 114:65–72.PubMedCrossRefGoogle Scholar
  11. Darnell, J., 1982, Variety in the level of gene control in eucaryotic cells, Nature 297:365–371.PubMedCrossRefGoogle Scholar
  12. Fiddes, J. C., and Goodman, H. M., 1980, The cDNA for the ß-subunit of human chorionic gonadotropin suggests evolution by gene by read-through into the 3’-untranslated region, Nature 286:684–687.PubMedCrossRefGoogle Scholar
  13. Fiddes, J. C., and Goodman, H. M., 1981, The gene encoding the common alpha subunit of the four human glycoprotein hormones, J. Molec. App. Gen. 1:3–18.Google Scholar
  14. Fiddes, J. C., and Talmadge, K., 1984, Structure, expression, and evolution of the genes for the human glycoprotein genes, Recent Prog. Horm. Res. 40:43–78.PubMedGoogle Scholar
  15. Gharib, S. D., Bowers, S. M., Need, L. R., and Chin, W. W., 1986, Regulation of rat luteinizing hormone (LH) subunit mRNAs by gonadal-steroid hormones, J. Clin. Invest. 77:582–589.PubMedCrossRefGoogle Scholar
  16. Godine, J. E., Chin, W. W., and Habener, J. F., 1982, A subunit of rat pituitary glycoprotein hormones. Primary structure of the precursor determined from the nucleotide sequence of cloned DNAs, J. Biol. Chem. 257:8368–8371.PubMedGoogle Scholar
  17. Goodwin, R. G., Moncman, C. L., Rottman, F. M., and Nilson, J. H., 1983, Characterization and nucleotide sequence of the gene for the common a-subunit of the bovine pituitary glycoprotein hormones, Nucleic Acids Res. 11:6873–6882.PubMedCrossRefGoogle Scholar
  18. Hayashizaki, Y., Miyai, K., Kato, K., and Matsubara, K., 1985, Molecular cloning of the human thyrotropin-ß subunit gene, FEBS Lett. 188:394–400.PubMedCrossRefGoogle Scholar
  19. Jameson, J. L., Chin, W. W., Hollenberg, A. N., Chang, A. S., and Habener, J. F., 1984, The gene encoding the 6-subunit of rat luteinizing hormone: Analysis of gene structure and evolution of nucleotide sequence, J. Biol. Chem. 259:15474–15480.PubMedGoogle Scholar
  20. Jonassen, J. A., and Roberts, J. L., 1984, Transcription of the rat beta LH gene is stimulated by GnRH, 14th Annual Meeting of the Society for Neuroscience Abstract #308.4.Google Scholar
  21. Julier, C., Weil, D., Couillin, P., Cote, J., Nguyen, V. C., Foubert, C., Boue, A., and Junien, C., 1984, The beta chorionic gonadotropinbeta luteinizing hormone gene cluster maps to human chromosome 19, Hum. Genet. 67:174–177.PubMedCrossRefGoogle Scholar
  22. Kalra, P. S., and Kalra, S. P., 1985, Control of gonadotropin secretion, in: “The Pituitary Gland”, H. Imura, ed., Raven Press, New York, pp. 189–220.Google Scholar
  23. Keutmann, H. T., Mcllroy, P. J., Bergert, E. R., and Ryan, R. J., 1983, Chemically deglycosylated human chorionic gonadotropin subunits: Characterization and biological properties, Biochemistry 22:3067–3072.PubMedCrossRefGoogle Scholar
  24. Kourides, I. A., Barker, P. E., Gurr, J. A., Pravtcheva, D. D., and Ruddle, F. H., 1984, Assignment of the genes for the a and ß subunits of thyrotropin to different mouse chromosomes, Proc. Natl. Acad. Sci. USA 81:517–519.PubMedCrossRefGoogle Scholar
  25. Landefeld, T. D., Kepa, J., and Karsch. F. J., 1983, Regulation of a subunit synthesis by gonadal steroid feedback in the sheep anterior pituitary, J. Biol. Chem. 258:2390–2393.PubMedGoogle Scholar
  26. Landefeld, T. D., and Kepa, J., 1984, Regulation of LH beta subunit mRNA in the sheep pituitary gland during different feedback states of estradiol, Biochem. Biophys. Res. Comm. 122:1307–1313.PubMedCrossRefGoogle Scholar
  27. Landefeld, T.D., Kepa, J., and Karsch, F., 1984, Estradiol feedback effects on the a-subunit mRNA in the sheep pituitary gland: Correlation with serum and pituitary luteinizing hormone concentrations, Proc. Natl. Acad. Sci. 81:1322–1326.PubMedCrossRefGoogle Scholar
  28. Landefeld, T., Kaynard, A., and Kepa, J., 1985, Pituitary a-subunit messenger ribonucleic acid remains elevated during the latter stages of the preovulatory luteinizing hormone surge, Endocrinology 117:934–938.PubMedCrossRefGoogle Scholar
  29. Mason, A. J., Hayflick, J. S., Long, N., Esch, F., Ueno, N., Ying, S.-Y., Guillemin, R., Niall, H., and Seeburg, P., 1985, Complementary cDNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-8, Nature 318:659–663.PubMedCrossRefGoogle Scholar
  30. Moore, W. T., Jr., Burleigh, B. D., and Ward, D. N., 1980, Chorionic gonadotropin; Comparative studies and comments on relationships to other glycoprotein hormones, in: “Chorionic Gonadotropins,” S. J. Segal, ed., Plenum Press, New York, pp. 89–126.CrossRefGoogle Scholar
  31. Naylor, S. L., Chin, W. W., Goodman, H. M., Lalley, P. A., Grzeschik, K. H., and Sakaguchi, A. Y., 1983, Chromosome assignment of genes encoding the a and 8 subunits of glycoprotein hormone in man and mouse, Som. Cell. Gen. 9:757–770.CrossRefGoogle Scholar
  32. Naylor, S. L., Sakaguchi, A. Y., McDonald, L., Todd, S., and Lalley, P. A., 1986, Mapping the thyrotropin ß subunit gene in man and mouse, Som. Cell. Genet., in press.Google Scholar
  33. Nilson, J. H., Nejedlik, M. T., Virgin, J. B., Crowder, M. E., and Nett, T. M., 1983, Expression of a subunit and luteinizing hormone 8 genes in the ovine anterior pituitary. Estradiol suppresses accumulation of mRNAs for both a subunit and luteinizing hormone 8, J. Biol. Chem., 258:12087–12090.PubMedGoogle Scholar
  34. Parsons, T. F., and Pierce, J. G., 1984, Free alpha-like material from bovine pituitaries: Removal of its 0-linked oligosaccharide permits combination with lutropin-beta, J. Biol. Chem. 259:2662–2666.PubMedGoogle Scholar
  35. Pierce, J. G., and Parsons, T. F., 1981, Glycoprotein hormones: Structure and function, Annu. Rev. Biochem. 50:465–495.PubMedCrossRefGoogle Scholar
  36. Policastro, P., Ovitt, C. D., Hoshina, M., Fukuoka, H., Boothby, M. R., and Boime, I., 1983, The subunit of human chorionic gonadotropin is encoded by multiple genes, J. Biol. Chem. 258:11492–11499.PubMedGoogle Scholar
  37. Sairam, M. R., and Bhargavi, G. N., 1985, A role for glycosylation of the alpha subunit in transduction of biological signal in glycoprotein hormones, Science 229:65–67.PubMedCrossRefGoogle Scholar
  38. Shupnik, M. A., Chin, W. W., and Ridgway, E. C., 1986, T3 regulation of thyrotropin subunit genes, in: “Mechanism of Action of Thyroid Hormone”, R. Tolman and L. DeGroot, eds., in press.Google Scholar
  39. Shupnik, M. A., Chin, W. W., Habener, J. F., and Ridgway, E. C., 1985, Transcriptional regulation of the thyrotropin subunit genes by thyroid hormones, J. Biol. Chem. 260:2900–2903.PubMedGoogle Scholar
  40. Stewart, M., and Stewart, F., 1977, Constant and variable regions in glycoprotein beta subunit sequences: Implications for receptor binding specificity, J. Mol. Biol., 116:175–179.PubMedCrossRefGoogle Scholar
  41. Talmadge, K., Boorstein, W. R., and Fiddes, J. C., 1983, The human genome contains seven genes for the ß-subunit of chorionic gonadotropin but only one gene for the 8-subunit of luteinizing hormone, DNA, 2:279–287.CrossRefGoogle Scholar
  42. Talmadge, K., Vamvakopoulos, N. C., and Fiddes, J. C., 1984, Evolution of the genes for the ß subunits of human chorionic gonadotropin and luteinizing hormone, Nature 307:37–40.PubMedCrossRefGoogle Scholar
  43. Tepper, M. A., Dionne, F. R., Eberwine, J. H., Wilcox, J. N., and Roberts, J. L., 1984, Regulation of beta LH gene expression during the estrous cycle and castration in the rat, 7th International Congress of Endocrinology Quebec City, Canada, Abstract #2293.Google Scholar
  44. Tepper, M. A., and Roberts, J. L., 1984, Evidence for only one ß luteinizing and no ß chorionic gonadotropin gene in the rat, Endocrinology 115:385–391.PubMedCrossRefGoogle Scholar
  45. Virgin, J. B., Silver, B. J., Thomason, A. R., and Nilson, J. H., 1985, The gene for the ß subunit of bovine luteinizing hormone encodes a gonadotropin mRNA with an unusually short 5’-untranslated region, J. Biol. Chem. 260:7072–7077.PubMedGoogle Scholar
  46. Watkins, P., Eddy, R., Beck, A., Vellucci, V., Gusella, J., and Shows, T., 1985, Assignment of the human gene for the ß subunit of follicle-stimulating hormone (FSHB) to chromosome 11, Cytogenet. Cell Genet. 40:773.CrossRefGoogle Scholar
  47. Weintraub, B. D., Stannard, B. S., and Meyers, L., 1983, Glycosylation of thyroid-stimulating hormone in pituitary tumor cells: Influence of high mannose oligosaccharide units on subunit aggregation, combination and intracellular degradation, Endocrinology 112:1331–1345.PubMedCrossRefGoogle Scholar
  48. Whitfield, G. K., Powers, R. E., Gurr, J. A., Wolf, O., and Kourides, I. A., 1985, Isolation of a gene encoding human thyrotropin beta subunit, 9th Intl. Thyroid Cong. Sao Paulo, Brazil, Abstract #14.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • William W. Chin
    • 1
    • 2
  • Soheyla D. Gharib
    • 1
    • 2
  1. 1.Department of Medicine, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA
  2. 2.Howard Hughes Medical Institute LaboratoriesHarvard Medical SchoolBostonUSA

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