Abstract
We have previously demonstrated that the Na+:Ca2+ exchanger found by Gmaj et al (1) in rat renal cortical basolateral membrane vesicles (BLMV) is present in canine renal cortical BLMV (2). We showed that its activity is depressed in the thyroparathyroidectomized (TPTX) state and that it was restored by in vivo infusion of parathyroid hormone (PTH). This effect was achieved by a change in Vmax of the exchanger but no significant change in the Km. It has been established that the phosphaturic effect of PTH on the proximal tubule can be mimicked by cAMP analogues (3). We sought to examine the effect of dBcAMP on the activation of the Na+:Ca2+ exchanger in BLMV prepared from TPTX dogs. The dose of dBcAMP used was chosen to achieve the same phosphaturic effect as the previously used 2 µg/Kg PTH (4).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
P. Gmaj, H. Murer, and R. Kinne, Calcium ion transport across plasma membranes isolated from rat kidney cortex, Biochem J. 178: 549–557, 1979.
J. E. Scoble, S. Mills, and K. A. Hruska, Calcium transport in canine renal basolateral membrane vesicles, J. Clin. Invest. 75: 1996–1105, 1985.
Z. S. Agus, J. B. Puschett, D. Senesky, and M. Goldberg, Mode of action of parathyroid hormone and cyclic adenosine 3’,5’-monophosphate on renal tubular phosphate reabsorption in the dog, J. Clin. Invest. 50: 617–626, 1971.
M. R. Hammerman, I. E. Karl, and K. A. Hruska, Regulation of canine renal vesicle Pi transport by growth hormone and parathyroid hormone, Biochim. Biophys. Acta 603: 322–335, 1980.
F. Morel, Sites of hormone action in the mammalian nephron, Amer. J. Physiol. 240: F159 - F164, 1981.
K. A. Hruska, S. Mills, and S. Westbrook, Regulation of proximal tubular (PT) cell cystosolic (Ca2+) by parathyroid hormone (PTH), Kidney Int. 27: 117, 1985.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Scoble, J.E., Moskowitz, D., Hruska, K.A. (1986). Dibutryladenosine 3’, 5’-Cyclic Monophosphate (dBcAMP) does not Mimic the Action of Parathyroid Hormone (PTH) on Canine Proximal Tubular Basolateral Membrane Na+:Ca2+ . In: Massry, S.G., Olmer, M., Ritz, E. (eds) Phosphate and Mineral Homeostasis. Advances in Experimental Medicine and Biology, vol 208. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5206-8_67
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5206-8_67
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5208-2
Online ISBN: 978-1-4684-5206-8
eBook Packages: Springer Book Archive