Regulation of Na+/H+ Antiport in the Intact Renal Proximal Tubular Cell

  • Edward P. Nord
  • Dalia Goldfarb
  • Leon G. Fine
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 208)


The kinetic properties of the Na+/H+ antiporter, present in the brush border membrane of the renal proximal tubule, have been delineated in a series of studies on isolated brush border membrane vesicles (1,2). In order to determine the factors which regulate this transport system using this approach, the system has had to be perturbed either in vivo or in vitro prior to fragmenting the cell for preparation of isolated vesicles. Whether disruption of the cell alters the apparent response to exogenous stimuli; e.g. hormones, neurogenic influences, etc., is not clear.


Brush Border Membrane Proximal Tubular Cell Transport Buffer Sodium Influx Proximal Tubular 
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  1. 1.
    J.L. Kinsella and P.S. Aronson, Properties of the Na+-H+ exchanger in renal microvillar membrane vesicles. Am J Physiol 238 (Renal Fluid Electrolyte Physiol 7): F461 - F469 (1980).Google Scholar
  2. 2.
    E.P. Nord, A. Hafezi, E.M. Wright, and L.G. Fine, Mechanisms of Na+ uptake into renal brush border membrane vesicles, Am J Physiol (Renal Fluid Electrolyte Physiol 16 ): F548 - F554 (1984).Google Scholar
  3. 3.
    E.P. Nord, D. Goldfarb, N. Mikhail, P. Moradeshaghi, A. Hafezi, S. Vaystub, E.J. Cragoe Jr., and L.G. Fine, Characteristics of the Na+-H antiporter in the intact renal proximal tubular cell, Am J Physiol (in press) (1985).Google Scholar
  4. 4.
    L.M. Sakhrani, B. Badie-Dezfooly, W. Trizna, N. Mikhail, A.G. Lowe, M. Taub, and L.G. Fine, Transport and metabolism of glucose in renal proximal tubular cells in primary culture. Am J Physiol 246 (Renal Fluid Electrolyte Physiol 15): F757 - F764 (1984).Google Scholar
  5. 5.
    P.A. Insel, M.D. Snavely, D.P. Healy, P.A. Munzel, C.L. Potenza, and E.P. Nord, Radioligand binding and functional assays demonstrate postsynaptic alpha2-receptors on proximal tubules of rat and rabbit kidney, J Cardiovasc Pharm (in press) (1985).Google Scholar
  6. 6.
    L.G. Fine, B. Badie-Dezfooly, A.G. Lowe, A. Hamzeh, J. Wells, and S. Salehmoghaddam, Stimulation of Na+/H+ antiport is an early event in hypertrophy of renal proximal cells. Proc Natl Acad Sci USA 82: 1736–1740 (1985).CrossRefGoogle Scholar
  7. 7.
    V.L. Schuster, J.P. Kokko, and H.R. Jacobson, Angiotensin II directly stimulates sodium transport in rabbit proximal convoluted tubules, J Clin Invest 73: 507–515 (1984).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Edward P. Nord
    • 1
  • Dalia Goldfarb
    • 1
  • Leon G. Fine
    • 1
  1. 1.Department of Medicine/Division of NephrologyUCLA School of MedicineLos AngelesUSA

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