Renal Adenylate Cyclase Stimulating Action of Normal and Oxidized Parathyroid Hormone (1–34)

  • J. J. Helwig
  • N. Burgmeier
  • A. Gairard
  • M. C. M. Yang
  • P. K. T. Pang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 208)


Parathyroid hormone (PTH) has a number of actions which can be demonstrated in vivo and in vitro assays. It has been shown to be hypercalcemic, hypocalciuric, hypophosphatemic, hyperphosphaturic, renal cortical membrane adenylate cyclase stimulating, renal 1-hydroxylase stimulating, and bone calcium mobilizing (see review by Parsons, 1979). Recently, PTH has also been studied extensively for its action on contraction of various smooth muscles. Although it has been repeatedly reported that PTH is hypotensive or vasodilating (Collip & Clark, 1925; Handler & Cohn, 1952; Charbon, 1966; Charbon & Hulstaert, 1974), recent studies from various laboratories have firmly established the vasorelaxing action of PTH (Berthelot & Gairard, 1975; Schleiffer et al., 1979; Pang et al., 1980a; Pang et al., 1985). In addition, the relaxing effect of PTH has been demonstrated in the uterus (Pang et al., 1981; Shew & Pang, 1984a), vas deferens (Zhang et al., 1985), trachea (Yen et al., 1983) and the gastrointestinal tract (Yang et al., 1981). Such a great variety of PTH actions then lead to the question as to whether the receptors are the same in all these target tissues and whether these actions are mediated by the same molecular structural component of the peptide hormone. Some previous attempts to answer such questions will be summarized below. We have recently begun a series of investigations on the adenylate cyclase stimulating action of PTH in rabbit renal microvessel, and tubule preparations. These data will be discussed here in relation to the question raised above.


Parathyroid Hormone Adenylate Cyclase Japanese Quail Adenylate Cyclase Stimulate Parathyroid Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aubach, G.D. and Chase, L.R., 1976, Cyclic nucleotides and biochemical actions of PTH and calcitonin. In: Handbook of Physiology, Endocrinology, vol. VII, Parathyroid Gland (Aubach, G.D., ed. ) p. 344.Google Scholar
  2. Berthelot, A. and Gairard, A., 1975, Effet de la parathormone sur la pression arterielle et la contraction de l’aorte isolee. Experientia, 31: 457.CrossRefGoogle Scholar
  3. Charbon, G.A., 1966, A diuretic and hypotensive action of a parathyroid extract. Acta Physiol. Pharmac. Neerl. 14: 52.Google Scholar
  4. Charbon, G.A. and Hulstaert, P.F., 1974, Augmentation of arterial hepatic and renal flow by extracted and synthetic parathyroid hormone. Endocrinol., 95: 621.CrossRefGoogle Scholar
  5. Collip, J.B. and Clark, E.P., 1925, Further studies on physiological action of parathyroid hormone. J. Biol. Chem., 64: 133.Google Scholar
  6. Dacke, C.G. and Kenny, A.D., 1973, Avian bioassay method for parathyroid hormone. Endocrinol., 92: 463.CrossRefGoogle Scholar
  7. Galceran, T., Lewis-Finch, J., Martin, K.J. and Slatopolsky, E., 1984, Absence of biological effects of oxidized parathyroid hormone-(1–34) in dogs and rats, Endocrinol., 115: 2375.CrossRefGoogle Scholar
  8. Handler, P. and Cohn, D.V., 1952, Effect of parathyroid extract on renal function. Am. J. Physiol., 169: 188.Google Scholar
  9. Helwig, J.J., Judes, C., Bollack, C. and Mandel, P., 1984a, Isolation and characterization of a microvascular fraction from rabbit kidney cortex. Renal Physiol., 7: 146.Google Scholar
  10. Helwig, J.J., Judes, C., Gairard, A. and Bollack, C., 1984b, J. Physiol. (Paris), 79: 166.Google Scholar
  11. Helwig, J.J., Schleiffer, R., Judes, C. and Gairard, A., 1984c, Distribution of parathyroid hormone-sensitive adenylate cyclase in isolated rabbit renal cortex microvessels and glomeruli. Life Sci., 35: 2649.CrossRefGoogle Scholar
  12. Kenny, A.D. and Pang, P.K.T., 1981, Dissociation of the hypercalcemic and hyperphosphaturic actions of bovine parathyroid hormome (1–34). Fed. Proc., 40: 399 (Abstract).Google Scholar
  13. Kenny, A.D. and Pang, P.K.T., 1982a, Structure activity relationship of parathyroid hormone: effects of oxidation. In: C. Oguro and P.R.T. Pang (eds.). Comparative Endocrinology of Calcium Regulation, Japanese Scientific Societies Press, Tokyo, p. 195.Google Scholar
  14. Kenny, A.D. and Pang, P.R.T., 1982b, Effect of oxidation on the urinary calcium response in rats to bovine parathyroid hormone. Calc. Tissue Int., 34: S41.Google Scholar
  15. Kenny, A.D. and Pang, P.K.T., 1982c, Response of the renal vitamin D endocrine system to oxidized parathyroid hormone (1–34). Proc. Soc. Exptl. Biol. Med., 171: 191.Google Scholar
  16. Pang, P.K.T., Tenner, T.E., Jr., Yee, J.A., Yang, M.C.M. and Janssen, H.F., 1980a, Hypotensive action of parathyroid hormone preparations on rats and dogs. Proc. Natn. Acad. Sci., USA, 77: 675.CrossRefGoogle Scholar
  17. Pang, P.K.T., Janssen, H.F. and Yee, J.A., 1980b, Effects of synthetic parathyroid hormone on vascular beds of dogs. Pharmacol., 21: 213.CrossRefGoogle Scholar
  18. Pang, P.K.T., Shew, R.L. and Sawyer, W.H., 1981, Inhibition of uterine contraction of synthetic parathyroid hormone fragment. Life Sci., 28: 1317.CrossRefGoogle Scholar
  19. Pang, P.K.T., Yang, M.C.M., Keutman, H.T. and Kenny, A.D., 1983, Structure activity relationship of parathyroid hormone: separation of the hypotensive and the hypercalcemic properties. Endocrinol., 112: 284.CrossRefGoogle Scholar
  20. Pang, P.K.T., Hong, B.S., Yen, L. and Yang, M.C.M., 1984, Parathyroid hormone. A specific potent vasodilator. Contri. Nephrol., 41: 137.Google Scholar
  21. Pang, P.K.T., Yang, M.C.M., Shew, R. and Tenner, T.E., Jr., 1985, The vasorelaxant action of parathyroid hormone fragments on isolated rat tail artery. Blood Vessels, 22: 57.Google Scholar
  22. Parsons, J.A., 1979, Physiology of PTH, Endocrinology, vol. 2, p. 621, edited by L.J. Degroot et al., Grune and Statton, N.Y.Google Scholar
  23. Potts, Jr., J.T., Aurbach, G.D. and Sherwood, L.M., 1966, Parathyroid hormone; chemical properties and structural requirements for biological and immunological activity. Recent Prog. Horm. Res., 22: 101.Google Scholar
  24. Rasmussen, H. and Craig, L.C., 1962, The parathyroid polypeptides. Recent. Prog. Horm. Res., 18: 269.Google Scholar
  25. Rosenblatt, M., Goltzman, D., Keutmann, H.T., Tregear, G.W. and Potts, Jr., J.T., 1976, Chemical and biological properties of synthetic, sulfur-free analogues of parathyroid hormone, J. Biol. Chem., 251: 159Google Scholar
  26. Schleiffer, R., Berthelot, A. and Gairard, A., 1979, Action of parathyroid extract on arterial blood pressure and on contraction and 45Ca exchange in isolated aorta or the rat. Evr. J. Pharmacol., 58: 163.Google Scholar
  27. Shew, R.L. and Pang, P.K.T., 1984a, Effects of bPTH fragments (1–34), (3–34) and (7–34) on bovine contraction. Peptides, 5: 485.CrossRefGoogle Scholar
  28. Shew, R.L., Kenny, A.D. and Pang, P.K.T., 1984b, Uterine relaxing action of parathyroid hormone: effect of oxidation and methionine substitution. Proc. Soc. Exp. Biol. Med., 175: 444.Google Scholar
  29. Tashjian, A.H., Ontjes, D.A. and Munson, P.L., 1964, Alkylation and oxidation of methionine in bovine parathyroid hormone: effects on hormonal activity and antigenicity. Biochemistry, 3: 1175.CrossRefGoogle Scholar
  30. Wang, H.H., Drugge, E.D., Yen, Y.C., Blumenthal, M.R. and Pang, P.K.T., 1984, Effect of synthetic parathyroid hormone on hemodynamics and regional blood flow. European J. Pharmacol., 97: 209.CrossRefGoogle Scholar
  31. Yang, M.C.M., Kenny, A.D. and Pang, P.R.T., 1984, Effect of parathyroid hormone on rat gastrointestinal contraction in vivo. Proc. IX Int. Symp. Comp. Endocrinol., Dec. 7–11, 1981, Hong Kong, in press.Google Scholar
  32. Yen, Y.C., Yang, M.C.M., Kenny, A.D. and Pang, P.K.T., 1983a, Parathyroid hormone (PTH) fragments relax the guinea pig trachea in vitro. Can. J. Physiol. Pharmacol., 61: 1324.CrossRefGoogle Scholar
  33. Yee, J.A., Shew, R.L. and Pang, P.K.T., 1983b, Effects of oxidized synthetic parathyroid hormone on bone in vitro. Calc. Tissue Int., 35: 44.CrossRefGoogle Scholar
  34. Zhang, R.H., Yang, M.C.M. and Pang, P.K.T., 1985, The relaxing effect of parathyroid hormone on rat vas deferens. Fed. Proc., 44: 1650.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • J. J. Helwig
    • 1
  • N. Burgmeier
    • 1
  • A. Gairard
    • 2
  • M. C. M. Yang
    • 3
  • P. K. T. Pang
    • 3
  1. 1.Centre de Recherches R. Leriche, Pav. Poincare, Hospices CivilsULPStrasbourgFrance
  2. 2.Laboratoire de Physiologie, INSERM U243-CNRS 787, Fac. PharmacieULPStrasbourgFrance
  3. 3.Dept. of PharmacologyTexas Tech University Health Sciences CenterLubbockUSA

Personalised recommendations