Renal Membrane Transport of Calcium

  • Yusuke Tsukamoto
  • Wadi N. Suki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 208)


The development of techniques for the isolation of highly purified baso-lateral membrane vesicles (BLMV) from the renal tubular epithelial cells enabled us to investigate the mechanism of calcium extrusion at this barrier of the cell. Two mechanisms of calcium transport known to exist in other tissues have been identified in the renal BLMV. These mechanisms are ATP-dependent Ca2+ transport and Na+/Ca2+ exchange. Yet, several aspects of these transporters are quite unique in the renal tubular cells.


Basolateral Membrane Kidney Cortex Renal Tubular Epithelial Cell Basolateral Membrane Vesicle Calcium Extrusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. Moore, D.F. Fitzpatrick, T.S. Chen, and E.J. Landon, Calcium pump activity of the renal plasma membrane and renal microsomes, Bioch. Biophys. Acta345: 405 (1974).CrossRefGoogle Scholar
  2. 2.
    P. Gmaj, H. Murer, and R. Kinne, Calcium ion transport across plasma membranes isolated from rat kidney cortex, Biochem. J. 178: 549 (1979).Google Scholar
  3. 3.
    B. Forbush, III, Characterization of right-side-out membrane vesicles rich in (Na,K)-ATPase and isolated from dog kidney outer medulla, J. Biol. Chem. 257: 12678 (1982).Google Scholar
  4. 4.
    P. Gmaj, M. Zurini, H. Murer, and E. Carafoli, A high-affinity, calmodulindependent Ca2+ pump in the basal-lateral plasma membranes of kidney cortex, Eur. J. Biochem. 136: 71 (1983).CrossRefGoogle Scholar
  5. 5.
    Z. Taylor, G. Richison, and J.A.L. Arruda, High-affinity calcium binding sites in luminal and basolateral renal membranes, Am. J. Physiol. 248: F472 (1985).Google Scholar
  6. 6.
    K.A. Hruska, S.C. Mills, S. Khalifa, and M.R. Hammerman, Phosphorylation of renal brush-border membrane vesicles, J. Biol. Chem. 258: 2501 (1983).Google Scholar
  7. 7.
    V. Niggli, E.S. Adunyah, J.T. Penniston, and E. Carafoli, Purified (Ca2+MgL+)-ATPase of the erythrocyte membrane, J. Biol. Chem. 256: 395 (1981).Google Scholar
  8. 8.
    P. Caroni, and E. Carafoli, The Ca2+ pumping ATPase of heart sarcolemma, J. Biol. Chem. 256: 3263 (1981).Google Scholar
  9. 9.
    Y. Tsukamoto, and B. Sacktor, Characterization of Ca2+-dependent ATPase in basolateral membrane of rat renal cortex: Regulatory role of 1,25(OH)2D3, Kidney Int. 25: 156 (1984).Google Scholar
  10. 10.
    A. Jayakumar, L. Cheng, C.T. Liang, and B. Sacktor, Sodium gradient-. dependent calcium uptake in renal basolateral membrane vesicles, J. Biol. Chem. 259: 10827 (1984).Google Scholar
  11. 11.
    P. (rmal, I-f. Murer, and E. Carafoli, Localization and properties of a high-affinity (Ca2+ + Mg2+)-ATPase in isolated kidney cortex plasma membranes, FEBS letters144: 226 (1982).CrossRefGoogle Scholar
  12. 12.
    W. Ghijsen, P. Gmaj, and H. Murer, Ca2+-stimulated Mg2+-pumping ATPase, Biochem. Biophys. Acta778: 481 (1984).CrossRefGoogle Scholar
  13. 13.
    Y. Tsukamoto, and W.N. Suki, Regulation of Ca2+-ATPase in the basolateral membrane of rat kidney cortex, Kidney Int. 27: 128 (1985).Google Scholar
  14. 14.
    S. Khalifa, S. Mills, and K.A. Hruska, Stimulation of calcium uptake by parathyroid hormone in renal brush-border membrane vesicles, J. Biol. Chem. 258: 14400 (1983).Google Scholar
  15. 15.
    K.J. Ullrich, G. Rumrich, and S. Kloss, Active Ca2+ reabsorption in the proximal tubule of the rat kidney. Dependence on sodium and buffer transport, Pflugers Arch. Eur. J. Physiol. 364: 223 (1976).CrossRefGoogle Scholar
  16. 16.
    K.D. Philipson, and A.Y. Nishimoto, Na+-Ca2+ exchange in inside-out cardiac sarcolemmal vesicles, J. Biol. Chem. 257: 5111 (1982).Google Scholar
  17. 17.
    J.E. Scoble, S. Mills, and K.A. Hruska, Calcium transport in canine renal basolateral membrane vesicles. Effect of parathyroid hormones, J. Clin. Invest. 75: 1096 (1985).CrossRefGoogle Scholar
  18. 18.
    H.S. Chase, Jr., Does calcium couple the apical and basolateral membrane permeabilities in epithelia ? Am. J. Physiol. 247: F869 (1984).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Yusuke Tsukamoto
    • 1
    • 2
  • Wadi N. Suki
    • 1
    • 2
  1. 1.Department of MedicineKitasato University School of MedicineJapan
  2. 2.Renal SectionBaylor College of MedicineHoustonUSA

Personalised recommendations