The Direct Effect of 1,25-Dihydroxycholecalciferol on Membrane Phospholipid Composition and Phosphate Transport

  • B. R. C. Kurnik
  • M. Huskey
  • K. A. Hruska
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 208)


Current evidence suggests that the mechanism of action of several hormones may be mediated through alterations in membrane lipid composition1–4. Both the content and the degree of fatty acid unsaturation of phosphatidylcholine (PC) in renal proximal tubular and duodenal brush border membranes is increased after 1,25-dihydroxycholecalciferol (1,25(OH)2D3) administration to animals5,6. In both the intestine57 and the kidney8 indirect evidence suggests that these lipid changes may be important in stimulating calcium and phosphate transport. In recent studies performed in this laboratory fluorescent phospholipid derivatives have been used to demonstrate that 1,25(OH)2D3 rapidly and directly stimulates the transfer of PC from liposomes to renal brush border membrane vesicles (BBMV)9. The following studies were performed to investigate the effects of 1,25(OH)2D3 induced membrane phospholipid alterations on phosphate transport.


Brush Border Membrane Phosphate Transport Phosphate Uptake Brush Border Membrane Vesicle Renal Brush Border Membrane 
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  1. 1.
    R.V. Farese, D.E. Barnes, J.S. Davis, M.L. Standaert, and R.J. Pallet, Effects of insulin and protein synthesis inhibitors on phospholipid metabolism, diaclyglycerol levles, and pyruvate dehydrogenase activity in BC3H-1 cultured myocytes, J. Biol. Chem. 259: 7094 (1984).Google Scholar
  2. 2.
    D. Marver, and J.P. Kokko, Renal target sites and the mechanism of action of aldosterone, Miner. & Electrolyte Metab. 9: 1 (1983).Google Scholar
  3. 3.
    I. Litosch, S.H. Lin, and J.N. Fain, Rapid changes in hepatocyte phosphoinositides induced by vasopressin, J. Biol. Chem. 258: 13727 (1983).Google Scholar
  4. 4.
    V. Meltzer, S. Weinreb, E. Bellorin-Font, and K.A. Hruska, Parathyroid hormone stimulation of renal phosphoinositide metabolism is a cyclic nucleotide-independent effect. Biochim. Biophys. Acta 712: 258 (1982).Google Scholar
  5. 5.
    M. Tsutsumi, U. Alvarez, L.V. Avioli, and K.A. Hruska, The effect of 1,25(OH)2D3 on phospholipid composition of rat renal brush border membrane, Am. J. Physiol. 249: (1985) in press.Google Scholar
  6. 6.
    T. Matsumoto, 0. Fontaine, and H. Rasmussen, Effect of 1,25-dihydroxy vitamin D3 on phospholipid metabolism in chick duodenal mucosal cell, J. Biol. Che. 256: 3354 (1981).Google Scholar
  7. 7.
    T. Matsumoto, O. Fontaine, and H. Rasmussen, Effect of 1,25-dihydroxy vitamin D3 on phosphate uptake into chick intestinal brush border membrane vesicles, Biochim. Biophys. Acta 599: 13 (1980).CrossRefGoogle Scholar
  8. 8.
    B.R.C. Kurnik, and K.A. Hruska, Mechanisms of stimulation of renal phosphate transport by 1,25-dihydroxycholecalciferol (Biochim. Biophys. Acta - in press).Google Scholar
  9. 9.
    B.R.C. Kurnik, and K.A. Hruska, Effects of 1,25(OH)2D3 directly stimulates phosphatidylcholine (PC) transfer, Kidney Int. 27: 119 (1985).Google Scholar
  10. 10.
    B.R.C. Kurnik, and K.A. Hruska, Effects of 1,25-dihydroxycholecalciferol on phosphate transport in vitamin D-deprived rats, Am. J. Physiol. 247: F177 (1984).Google Scholar
  11. 11.
    B.R.C. Kurnik, M. Huskey, D. Hagerty, and K.A. Hruska, Vitamin D metabolites stimulate phosphatidylcholine transfer to renal brush border membranes. Submitted to J. Biol. Chem., May 1985.Google Scholar
  12. 12.
    D.K. Struck, and R.E. Pagano, Insertion of fluorescent phospholipids into the plasma membrane of a mammalian cell, J. Biol. Chem. 255: 5404 (1980).Google Scholar
  13. 13.
    E.G. Bligh, and W.J. Dyer, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol. 37: 911 (1959).CrossRefGoogle Scholar
  14. 14.
    B.N. Ames, and D.T. Dubin, The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid, J. Biol. Chem. 235: 769 (1960).Google Scholar
  15. 15.
    R. Welti, and G.M. Helmkamp, Jr., Acyl chain specificity of phosphatidylcholine transfer protein from bovine liver, J. Biol. Chem. 259: 6937 (1984).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • B. R. C. Kurnik
    • 1
  • M. Huskey
    • 1
  • K. A. Hruska
    • 1
  1. 1.Renal DivisionWashington University and The Jewish Hospital of St. LouisSt. LouisUSA

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