The Direct Effect of 1,25-Dihydroxycholecalciferol on Membrane Phospholipid Composition and Phosphate Transport

  • B. R. C. Kurnik
  • M. Huskey
  • K. A. Hruska
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 208)


Current evidence suggests that the mechanism of action of several hormones may be mediated through alterations in membrane lipid composition1–4. Both the content and the degree of fatty acid unsaturation of phosphatidylcholine (PC) in renal proximal tubular and duodenal brush border membranes is increased after 1,25-dihydroxycholecalciferol (1,25(OH)2D3) administration to animals5,6. In both the intestine57 and the kidney8 indirect evidence suggests that these lipid changes may be important in stimulating calcium and phosphate transport. In recent studies performed in this laboratory fluorescent phospholipid derivatives have been used to demonstrate that 1,25(OH)2D3 rapidly and directly stimulates the transfer of PC from liposomes to renal brush border membrane vesicles (BBMV)9. The following studies were performed to investigate the effects of 1,25(OH)2D3 induced membrane phospholipid alterations on phosphate transport.


Brush Border Membrane Phosphate Transport Phosphate Uptake Brush Border Membrane Vesicle Renal Brush Border Membrane 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • B. R. C. Kurnik
    • 1
  • M. Huskey
    • 1
  • K. A. Hruska
    • 1
  1. 1.Renal DivisionWashington University and The Jewish Hospital of St. LouisSt. LouisUSA

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