Choline Fluxes to and from the Rat Cerebral Cortex Studied with the “Cup Technique” in Vivo

  • R. Corradetti
  • R. Brehm
  • K. Löffelholz
  • G. Pepeu
Part of the Advances in Behavioral Biology book series (ABBI, volume 30)


Since MacIntosh and Oborin (11) and later Mitchell (12) introduced the “cup technique” as a mean to study acetylcholine release from the cerebral cortex in vivo, this technique has been widely used for investigating the release of various neurotransmitters in anaesthetized as well as unanaesthetized mammals (2, 13, 14). Recently we proposed the “cup technique” as a way for studying the efflux of endogenous choline (Ch) from the rat cerebral cortex (4, 5) and to estimate changes in the extracellular concentration of Ch, if we consider the fluid filling the cup as an extension of the extracellular space.


Ringer Solution Tracer Dose Chicken Heart Arachnoid Space Muscarinic Receptor Activation 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • R. Corradetti
    • 1
  • R. Brehm
    • 2
  • K. Löffelholz
    • 2
  • G. Pepeu
    • 1
  1. 1.Department of PharmacologyUniversity of FlorenceFlorenceItaly
  2. 2.Department of PharmacologyUniversity of MainzMainzFederal Republic Of Germany

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