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Choline Fluxes to and from the Rat Cerebral Cortex Studied with the “Cup Technique” in Vivo

  • R. Corradetti
  • R. Brehm
  • K. Löffelholz
  • G. Pepeu
Part of the Advances in Behavioral Biology book series (ABBI, volume 30)

Abstract

Since MacIntosh and Oborin (11) and later Mitchell (12) introduced the “cup technique” as a mean to study acetylcholine release from the cerebral cortex in vivo, this technique has been widely used for investigating the release of various neurotransmitters in anaesthetized as well as unanaesthetized mammals (2, 13, 14). Recently we proposed the “cup technique” as a way for studying the efflux of endogenous choline (Ch) from the rat cerebral cortex (4, 5) and to estimate changes in the extracellular concentration of Ch, if we consider the fluid filling the cup as an extension of the extracellular space.

Keywords

Ringer Solution Tracer Dose Chicken Heart Arachnoid Space Muscarinic Receptor Activation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bartus, R.T., Dean, R.L., Beer, B. and Lippa, S.S. (1982): Science 217: 408–417.CrossRefGoogle Scholar
  2. 2.
    Beani, L. and Bianchi, C. (1970): In Drugs and Cholinergic Mechanisms in the CNS (eds) E. Heilbronn and A. Winter, Res. Inst. of National Defense, Stockholm, pp. 369–386.Google Scholar
  3. 3.
    Cornford, E:M., Braun, L.D. and Oldendorf, W.H. (1978): J. Neurochem. 30: 299–308.Google Scholar
  4. 4.
    Corradetti, R., Lindmar, R. and Löffelholz, K. (1982): Europ. J. Pharmacol. 85: 123–124.CrossRefGoogle Scholar
  5. 5.
    Corradetti, R., Lindmar, R. and Lbffelholz, K. (1983): J. Pharmacol. Exp. Ther. 226: 826–832.Google Scholar
  6. 6.
    Dayson, H. (1976): J. Physiol. ( Lond. ) 255: 1–28.Google Scholar
  7. 7.
    Estrada, C., Hamel, E. and Krause, D.N. (1983): Brain Res. 266: 261–270.CrossRefGoogle Scholar
  8. 8.
    Goldberg, A.M. and McCaman, R.E. (1974): In Choline and Acetylcholine. Handbook of Chemical Assay Methods (ed) I. Hanin, Raven Press, New York, pp. 47–61.Google Scholar
  9. 9.
    Grammas, P., Diglio, C.A., Marks, B.H., Giacomelli, F. and Wiener, J. (1983): J. Neurochem. 40: 645–651.CrossRefGoogle Scholar
  10. 10.
    Isragl, M. and Lesbats, B. (1982): J. Neurochem. 39: 248–250.CrossRefGoogle Scholar
  11. 11.
    MacIntosh, F.C. and Oborin, P.E. (1953): Abstr. XIX Int. Physiol. Congr. pp. 580–581.Google Scholar
  12. 12.
    Mitchell, J.F. (1963): J. Physiol. ( Lond. ) 165: 98–116.Google Scholar
  13. 13.
    Moroni, F. and Pepeu, G. (1984): In IBRO Handbook Series: Methods in Neurosciences (ed) C.A. Marsden, John Wiley & Sons Ltd., Chichester, New York, pp. 63–79.Google Scholar
  14. 14.
    Pepeu, G. (1973): Prog. Neurobiol. 2: 257–288.CrossRefGoogle Scholar
  15. 15.
    Polak, R.L. (1971): Brit. J. Pharmacol. 41: 600–606.Google Scholar
  16. 16.
    Reed, D.J. and Woodbury, D.M. (1966): J. Physiol. ( Lond. ) 169: 816–850.Google Scholar
  17. 17.
    Steinwall, O. and Klatzo, I. (1966): J. Neuropathol. Exp. Neurol. 25: 542–559.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • R. Corradetti
    • 1
  • R. Brehm
    • 2
  • K. Löffelholz
    • 2
  • G. Pepeu
    • 1
  1. 1.Department of PharmacologyUniversity of FlorenceFlorenceItaly
  2. 2.Department of PharmacologyUniversity of MainzMainzFederal Republic Of Germany

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