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Biochemistry and Immunocytochemistry of Choline Acetyltransferase

  • P. M. Salvaterra
  • G. D. Crawford
  • C. R. Houser
  • D. A. Matthews
  • R. P. Barber
  • J. E. Vaughn
Part of the Advances in Behavioral Biology book series (ABBI, volume 30)

Abstract

Choline acetyltransferase (ChAT, acetyl CoA-choline-O-acetyltransferase, EC 2.3.1.6) is the enzyme responsible for catalyzing the synthesis of the important central and peripheral nervous system neurotransmitter, acetylcholine (ACh), and therefore the regulation of ChAT activity is likely to be a controlling factor in availability of ACh for neural transmission. Thus, many studies have been done to establish the relationship of ChAT activity to ACh levels as well as the factors regulating enzyme activity (for reviews see 9, 19, 23). In addition, ChAT may serve as a “phenotypic” marker protein for cells that make and use ACh as a neurotransmitter. Stimulated in large part by this latter possibility, many laboratories have attempted to produce specific antibodies for ChAT that would be useful in immunocytochemical procedures to study “cholinergic” pathways (1, 2, 8, 11, 14, 17, 26).

Keywords

Superior Cervical Ganglion Scale Line ChAT Activity Preganglionic Sympathetic Neuron Medial Septal Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • P. M. Salvaterra
    • 1
  • G. D. Crawford
    • 1
  • C. R. Houser
    • 1
  • D. A. Matthews
    • 1
  • R. P. Barber
    • 1
  • J. E. Vaughn
    • 1
  1. 1.Division of NeurosciencesBeckman Research Institute of the City of HopeDuarteUSA

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