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Storage and Secretion of Acetylcholine and Dopamine by PC12 Cells

  • B. D. Howard
  • W. P. Melega
Part of the Advances in Behavioral Biology book series (ABBI, volume 30)

Abstract

PC12 is a clonal line of rat pheochromocytoma cells that upon exposure to nerve growth factor extend neurites and acquire the morphology of neurons (7, 25). Both undifferentiated and nerve growth factor treated cells store and secrete dopamine and acetylcholine (9, 14, 16, 20, 23). This property makes PC12 useful for. pharmacological studies in that one can compare the effects of a particular drug on two different transmitter systems in the same cell.

Keywords

PC12 Cell Nerve Growth Factor Bovine Brain Preimmune Serum Motor Nerve Terminal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Amy, C.M. and Kirshner, N. (1981): J. Neurochem. 36: 847–854.CrossRefGoogle Scholar
  2. 2.
    Anderson, D.C., King, S.C. and Parsons, S.M. (1983): Molec. Pharmacol. 24: 48–54.Google Scholar
  3. 3.
    Baker, P.F. and Knight, D.E. (1979): Trends Neurosci. 2: 288–290.CrossRefGoogle Scholar
  4. 4.
    Bloom, F.E., Ueda, T., Battenberg, E. and Greengard, P. (1979): Proc. Natl. Acad. Sci. U.S.A. 76: 5982–5986.CrossRefGoogle Scholar
  5. 5.
    DeCamilli, P., Ueda, T., Bloom, F.E., Battenberg, E. and Greengard, P. (1979): Proc. Natl. Acad. Sci. U.S.A. 76: 5977–5981.CrossRefGoogle Scholar
  6. 6.
    DeLorenzo, R.J. and Freedman, S.D. (1977): Biochem. Biophys. Res. Commun. 77: 1036–1043.CrossRefGoogle Scholar
  7. 7.
    Greene, L.A. and Tischler, A.S. (1976): Proc. Natl. Acad. Sci. U.S.A. 73: 2424–2428.CrossRefGoogle Scholar
  8. 8.
    Greene, L.A. and Rein, G. (1977): Brain Res. 129: 247–263.CrossRefGoogle Scholar
  9. 9.
    Greene, L.A. and Rein, G. (1977): Nature 268: 349–351.CrossRefGoogle Scholar
  10. 10.
    Johansen, T. (1979): Eur. J. Pharmacol. 58: 107–115.MathSciNetCrossRefGoogle Scholar
  11. 11.
    Kirshner, N. and Smith, W.J. (1966): Science 154: 422–423.CrossRefGoogle Scholar
  12. 12.
    Krueger, B.K., Forn, T. and Greengard, P. (1977): J. Biol. Chem. 252: 2764–2773.Google Scholar
  13. 13.
    Laemmli, U.K. (1970): Nature 227: 680–685.CrossRefGoogle Scholar
  14. 14.
    Lee, K., Sabban, E., Goldstein, M., Seeleg, P.-J. and Greene, L.A. (1982): Neurosci. Soc. Abs. 8: 886.Google Scholar
  15. 15.
    Marshall, I.G. (1970): Br. J. Pharmacol. 38: 503–516.Google Scholar
  16. 16.
    Melega, W.P. and Howard, B.D. (1981): Biochemistry 20:4477–4483.CrossRefGoogle Scholar
  17. 17.
    Nelson-Krause, D.C. and Howard, B.D. (1978): Brain Res. 147: 91–105.CrossRefGoogle Scholar
  18. 18.
    Opperman, H., Levinson, A.D., Varmus, H.E., Levintow, L. and Bishop, J.M. (1979): Proc. Natl. Acad. Sci. U.S.A. 76: 1804–1808.CrossRefGoogle Scholar
  19. 19.
    Pawson, T., Guyden, J., Kunz, T.S., Radke, K., Gilmore, T. and Martin, G.S. (1980): Cell 122: 767–775.CrossRefGoogle Scholar
  20. 20.
    Rebois, R.V., Reynolds, E.E., Toll, L. and Howard, B.D. (1980): Biochemistry 19: 1240–1248.CrossRefGoogle Scholar
  21. 21.
    Reynolds, E.E., Melega, W.P. and Howard, B.D. (1982): Biochemistry 21: 4795–4799.CrossRefGoogle Scholar
  22. 22.
    Rubin, R.P. (1970): J. Physiol. 206: 181–192.Google Scholar
  23. 23.
    Schubert, D. and Klier, F.G. (1977): Proc. Natl. Acad. Sci. U.S.A. 74: 5184–5188.CrossRefGoogle Scholar
  24. 24.
    Sieghart, W., Theoharides, T.C., Alper, S.L., Douglas, W.W. and Greengard, P. (1978): Nature 275: 329–331.CrossRefGoogle Scholar
  25. 25.
    Tischler, A.S. and Greene, L.A. (1978): Lab. Invest. 39: 77–89.Google Scholar
  26. 26.
    Toll, L. and Howard, B.D. (1980): J. Biol. Chem. 255: 1787–1789.Google Scholar
  27. 27.
    Towbin, H., Staehlin, T. and Gordon, J. (1979): Proc. Natl. Acad. Sci. U.S.A. 76: 4350–4364.CrossRefGoogle Scholar
  28. 28.
    Ueda, T. and Greengard, P. (1977): J. Biol. Chem. 252: 5155–5163.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • B. D. Howard
    • 1
  • W. P. Melega
    • 1
  1. 1.Department of Biological Chemistry School of MedicineUniversity of California, Los AngelesLos AngelesUSA

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