Immunological Approach to Cholinergic Transmission: Production of Monoclonal Antibodies Against Presynaptic Membranes Isolated from the Electric Organ of Torpedo Marmorata
The release of the neurotransmitter acetylcholine (ACh) has been thoroughly analyzed with the techniques of electrophysiology. The events taking place at the membrane of the cholinergic nerve endings during ACh release were more recently visualized by morphological analysis of freeze fractured membranes. However, the biochemical basis of the neurotransmitter release is still poorly understood. A major question is which components of the membrane of the cholinergic nerve endings are involved in the release mechanism? One means of identifying some of these components would be to apply the monoclonal antibody strategy (13); in this way, it should be possible to isolate a monoclonal antibody with an exquisite specificity towards a given component. This antibody would then be used as a tool to purify and further characterize the antigen.
KeywordsSynaptic Vesicle High Specific Activity Electric Organ Presynaptic Membrane ChAT Activity
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