Regional Cerebral Blood Flow in Normal Blood Circulated and Perfluorocarbon Transfused Rats
Radiolabeled microspheres have been used routinely to measure regional blood flow and distribution of cardiac output in large animals such as dogs and sheep1,2,3 and the method has been applied more recently to smaller animals such as the rat.4,5,6 A critical requirement of this technique is that the microspheres are adequately mixed with the circulating blood volume after injection, therefore accurate coronary and cerebral blood flow measurements necessitate an atrial or ventricular injection site. The latter is usually accomplished by retrograde entry through the right carotide artery and left atrial cannulation is performed via thoracotomy or cardiac puncture. However, ventricular cannulation can be performed through the right brachial artery eliminating problems encountered with right carotid entry, i.e., left vs right differences, and any trauma associated with the thoracotomy or cardiac puncture. This procedure has been used previously in the rat to compare cerebral blood flow (CBF) measurements determined using microspheres with those obtained by the iodo-14C-antipyrine method (7).
KeywordsCerebral Blood Flow Regional Blood Flow Blood Substitute Organ Blood Flow Cerebral Blood Flow Measurement
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