Concurrent Measurements of O2 Partial Pressures and pH Values in Human Mammary Carcinoma Xenotransplants

  • Friedrich Kallinowski
  • Peter Vaupel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


Due to severe restrictions of convective and diffusive transport, hypoxic or even anoxic areas exist in malignant tumors. In addition, a high glycolytic rate in tumors both in the presence and absence of oxygen and the insufficient removal of the hereby produced lactic acid lead to an acidification of the tumor tissue. Both hypoxia and acidosis can influence the efficacy of irradiation, chemotherapy and hyperthermia. However, no comprehensive data on the development of both hypoxia and acidosis within the same tumor are available to date. In particular, there is no information regarding human tumors. Therefore, a new model has been developed which allows the systematic evaluation of both parameters in human tumor material in vivo under controlled systemic conditions. Due to its frequency and problematic clinical prognosis, cancer of the breast was chosen as the first tumor for investigation.


Medullary Carcinoma Anaplastic Carcinoma High Ammonia Concentration Tissue Oxygen Tension Human Mammary Carcinoma 
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  1. Ashby, B.S., 1966, pH studies in human malignant tumors, Lancet 2: 312.Google Scholar
  2. Badib, A.O., and Webster, J.H., 1969, Changes in tumor oxygen tension during radiation therapy, Acta radiol 8: 247.Google Scholar
  3. v.d. Berg, A.P., Wike-Hooley, J.L., v.d. Berg-Blok, A.E., v.d. Zee, J., and Reinhold, H.S., 1982, Tumour pH in human mammary carcinoma, Eur. J. Cancer Clin. Oncol. 18: 457.Google Scholar
  4. Busemeyer, J., Vaupel, P., and Thews, G., 1977, Diffusion coefficients of glucose in tumor tissue, Pfluegers Arch 368: R 17.Google Scholar
  5. Cater, D.B., and Silver, J.A., 1960, Quantitative measurements of oxygen tension in normal tissues and in tumours of patients before and after radiotherapy, Acta radiol 53: 233.Google Scholar
  6. Dave, S., Kallinowski, F., and Vaupel, P., in press, Blood flow and oxygen supply to human mammary carcinomas transplanted into nude rats, Adv. exp. Med. Biol.Google Scholar
  7. Evans, N.T.S., and Naylor, P.F.D., 1963, The effect of oxygen breathing and radiotherapy upon the tissue oxygen tension of some human tumors, Brit. J. Radiol. 36: 418.Google Scholar
  8. Grote, J., Suesskind, R., and Vaupel, P., 1977, Oxygen diffusivity in tumor tissue (DS- Carcinosarcoma) under temperature conditions within the range of 20–40 °C, Pfluegers Arch 372: 37.Google Scholar
  9. Hinsull, S.M., Colson, R.H., Franklin, A., Watson, B.W., and Bellamy, D., 1984, Determination of extracellular pH and tissue temperature in transplantable rat tumors by use of inductive loop telemetry, J. Natl. Cancer Inst. 73: 463.Google Scholar
  10. Inch, W.R., 1954, Direct current potential and pH of several varieties of skin neoplasms, Can. J. Biochem. Physiol. 32: 519.Google Scholar
  11. Jamieson, D., and v.d. Brenk, H.A.S., 1965, Oxygen tension in human malignant disease under hyperbaric conditions, Brit. J. Cancer 19: 139.Google Scholar
  12. Jain, R.K., Shah, S.A., and Finney, P.L., 1984, Continuous noninvasive monitoring of pH and temperature in rat Walker 256 carcinoma during normoglycemia and hyperglycemia, J. Natl. Cancer Inst. 73: 429.Google Scholar
  13. Kallinowski, F., Dave, S., Vaupel, P., Fortmeyer, H.P., Foerster, H., and Hoos, I., 1985, Modulation of ammonia detoxification in certain human tumors, Infusionstherapie 12: 22.Google Scholar
  14. Kallinowski, F., Dave, S., and Vaupel, P., in press, Glucose, lactate, and ketone body utilization by human mammary carcinomas in vivo, Adv. exp. Med. Biol.Google Scholar
  15. Kolstad, P., 1968, Intercapillary distance, oxygen tension and local recurrence in cervix cancer, Scand. J. clin. Lab. Invest. 22 (Suppl. 106): 145.Google Scholar
  16. Li, C.K.N., 1982, The glucose distribution in 9L rat brain multicell tumor spheroids and its effect on cell necrosis, Cancer 50: 2066.Google Scholar
  17. Naeslund, J., and Swenson, K.E., 1953, Investigations on the pH of malignant tumours in mice and humans after the administration of glucose, Acta Obstet. Gynecol. Scand. 32: 359.Google Scholar
  18. Pampus, F., 1963, Die Wasserstoffionenkonzentration des Hirngewebes bei raumfordernden intracraniellen Prozessen, Acta neurochir 11: 305.Google Scholar
  19. Pappova, N., Siracka, E., Vacek, A., and Durkovsky, J., 1982, Oxygen tension and prediction of the radiation response. Polarographic study in human breast cancer, Neoplasma 29: 669.Google Scholar
  20. Thistlethwaite, A.J., Leeper, D.B., Moylan, D.J., and Nerlinger, R.E., 1984, pH distribution in human tumors, Proc. 4th Ann. Meet. North American Hyperthermia Group Orlando, p. 137.Google Scholar
  21. Urbach, F., 1956, Pathophysiology of malignancy: I. Tissue oxygen tension of benign and malignant tumors of the skin, Proc. Soc. exp. Biol. Med. 92: 644.PubMedGoogle Scholar
  22. Urbach, F., and Noell, W.K., 1958, Effects of oxygen breathing on tumor oxygen measured polarographically, J. Appl. Physiol. 13: 61.PubMedGoogle Scholar
  23. Vaupel, P., 1974, Atemgaswechsel und Glucosestoffwechsel von Implantationstumoren (DS- Carcinosarkom) in vivo, Funktionsanalyse biologischer Systeme 1: 1.Google Scholar
  24. Vaupel, P., 1977, Hypoxia in neoplastic tissue, Microvasc. Res. 13: 399.PubMedCrossRefGoogle Scholar
  25. Vaupel, P., 1979, Oxygen supply to malignant tumors, in: “Tumor blood circulation: angiogenesis, vascular morphology and blood flow of experimental and human tumors,” Peterson, HI., ed., CRC Press, Boca Raton.Google Scholar
  26. Vaupel, P., 1982, Einfluß einer lokalisierten Mikrowellen-Hyperthermie auf die pH- Verteilung in bösartigen Tumoren, Strahlentherapie 158: 168.Google Scholar
  27. Vaupel, P., Frinak, S., Bicher, H.J., 1981, Heterogeneous oxygen partial pressure and pH distribution in C3H mouse mammary adenocarcinoma, Cancer Res 41: 2008.Google Scholar
  28. Whalen, W.J., Nair, P., and Ganfield, R.A., 1973, Measurements of oxygen tension in tissues with a micro oxygen electrode. Microvasc. Res. 5: 254.PubMedCrossRefGoogle Scholar
  29. Wike-Hooley, J.L., v.d. Zee, J., v. Rhoon, G.C., v.d. Berg, A.P., and Reinhold, H.S., 1984, Human tumour pH changes following hyperthermia and radiation therapy, Eur. J. Cancer Clin. Oncol. 20: 619.PubMedCrossRefGoogle Scholar
  30. Wike-Hooley, J.L., v.d. Berg, A.P., v.d. Zee, J., and Reinhold, H.S., in press, Human tumour pH and its variation, Eur. J. Cancer Clin. Oncol.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Friedrich Kallinowski
    • 1
  • Peter Vaupel
    • 1
  1. 1.Department of Applied PhysiologyUniversity of MainzMainzGermany

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