Use of the Oxidase Electrode to Determine Tumor Cell Metabolism of Peroxide
The purpose of the present study was to determine what factors govern cellular reduction of hydrogen peroxide. Both catalase and glutathione (GSH) peroxidase play a role in reducing peroxide, however, it has not been precisely determined to what degree each enzyme is involved. Therefore, we have studied the total capacity of two tumor cell lines to reduce peroxide as well as the contribution of each enzyme.
KeywordsA549 Cell Catalase Activity Peroxide Reduction Electrode Chamber Oxygen Monitor
Unable to display preview. Download preview PDF.
- 1.M. E. Varnes, S. Tuttle, and J. E. Biaglow, The use of a peroxidase electrode to measure the effects of nitro compounds on intact cells, in: “Oxygen Transport to Tissue — IV, ” H. Bicher and D. Bruley, eds., Plenum Publishing Co., New York (1983).Google Scholar
- 2.M. E. Varnes, J. E. Biaglow, L. Donahue, and S. Tuttle, Effects of glucose and thiol depletion on chemically—induced peroxide production in mammalian cells, in: “Oxygen Transport to Tissue — VI,” D. Bruley, H. Bicher, and D. Reneau, eds., Plenum Publishing Co., New York (1984).Google Scholar
- 4.YSI Instrument Co., Instructions for YSI Model 25 Oxidase Meter and YSI 2510 Oxidase Probe (1984).Google Scholar
- 5.A. Habeeb, Reactions of protein sulfhydryl groups with Eliman’s reagent, Methods in Enz. 25: 457 (1972).Google Scholar
- 6.F. Tietze, Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues, Anal. Biochem. 27:502 (1969).Google Scholar
- 7.V. Oyama and H. Eagle, Measurement of cell growth in tissue culture with a phenol reagent (Folin—Ciocalteau), Proc. Soc. Exp. Biol. Med. 91:305 (1956).Google Scholar
- 8.T. Yau and J. Mencl, A study of the peroxidation of fatty acid micelles promoted by ionizing radiation, hyrogen peroxide and ascorbate, Int. J. Radiat. Biol. 40:47 (1981).Google Scholar
- 9.J. E. Biaglow, M. E. Varnes, E. Clark, E. Epp, The role of thiols in cellular response to radiation and drugs, Radiat. Res. 95: 437 (1983).Google Scholar
- 10.M. E. Varnes, S. Tuttle, J. E. Biaglow, Nitroheterocycle metabolism in mammalian cells: Stimulation of the hexose monophosphate shunt, Biochem. Pharmacol. 33:1671 (1984).Google Scholar
- 11.L. Flohe and I. Brand, Kinetics of glutathione peroxidase, Biochim. Biophys. Acta. 191:541 (1969).Google Scholar