Use of the Oxidase Electrode to Determine Tumor Cell Metabolism of Peroxide

  • Laurie Donahue
  • John E. Biaglow
  • Marie E. Varnes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


The purpose of the present study was to determine what factors govern cellular reduction of hydrogen peroxide. Both catalase and glutathione (GSH) peroxidase play a role in reducing peroxide, however, it has not been precisely determined to what degree each enzyme is involved. Therefore, we have studied the total capacity of two tumor cell lines to reduce peroxide as well as the contribution of each enzyme.


A549 Cell Catalase Activity Peroxide Reduction Electrode Chamber Oxygen Monitor 


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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Laurie Donahue
    • 1
  • John E. Biaglow
    • 1
  • Marie E. Varnes
    • 1
  1. 1.Department of Radiology, Division of Biochemical OncologyCase Western Reserve UniversityClevelandUSA

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