Role of Cellular Non-Protein Thiols in Oxygen Consumption and Peroxide Reduction

  • Marie E. Varnes
  • John E. Biaglow
  • Stephen W. Tuttle
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


This study, and the study of Donahue et al., presented in the same volume (1), are continuations of our investigations into use of the Clark oxidase electrode (2) to monitor response of mammalian cells to peroxide and peroxide-producing drugs (3–5).


A549 Cell Pyridine Nucleotide Buthionine Sulfoximine Peroxide Reduction Diethyl Maleate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. Donahue, J. E. Biaglow and M. E. Varnes, Use of the oxidase electrode to determine tumor cell metabolism of peroxide, Adv. Exptl. Med. and Biol., this volume.Google Scholar
  2. 2.
    L. C. Clark, The hydrogen peroxide sensing platinum anode as an analytical enzyme electrode, Methods in Enzymol. 56: 448 (1979).Google Scholar
  3. 3.
    M. E. Varnes, J. E. Biaglow, L. Donahue and S. W. Tuttle, Effects of glucose and thiol depletion on chemically-induced peroxide production in mammalian cells, Adv. Exptl. Med. and Biol. 180: 663 (1984)Google Scholar
  4. 4.
    J.E.Biaglow,M.E.Varnes,M.Astor,J.Mitchell and A.Russo,Intracellular thiols:Involvement in drug metabolism and radiation response,in:“Radioprotectors and Anticarcinogens”,0.Nygaard and M.Simic,eds.,Academic Press,NY,p.203 (1983).Google Scholar
  5. 5.
    M. E. Varnes, S. W. Tuttle and J. E. Biaglow, The use of a peroxidase electrode to measure the effects of nitro compounds on intact cells, Adv. Exptl. Med. and Biol. 159: 49 (1983).Google Scholar
  6. 6.
    J. E. Biaglow, Cellular electron transfer and radical mechanisms for drug metabolism, Radiat. Res. 86: 212 (1981).Google Scholar
  7. 7.
    J. E. Biaglow, B. Jacobson, M. E. Varnes and C. Koch, The oxidation of ascorbate by electron affinic drugs and carcinogens, Photochem. Photobiol. 28: 869 (1978).Google Scholar
  8. 8.
    J. E. Biaglow, S. Tuttle, M. Varnes, E. Clark and E. Epp, Importance of glutathione, glutathione peroxidase, catalase and vitamin E in the aerobic cytotoxicity of misonidazole: Studies with A549 human lung carcinoma cells, Cancer Res.,submitted 1985.Google Scholar
  9. 9.
    J. E. Biaglow, M. E. Varnes, E. P. Clark and E. R. Epp, The role of thiols in cellular response to radiation and drugs, Radiat. Res. 95: 437 (1983).Google Scholar
  10. 10.
    J. B. Mitchell, A. Russo, J. E. Biaglow and S. McPherson, Cellular glutathione depletion of diethyl maleate or buthionine sulfoximine and its effects on the oxygen enhancement ratio, Radiat. Res. 94: 612 (1983).Google Scholar
  11. 11.
    B. Chance, H, Sies and A. Boveris, Hydroperoxide metabolism in mammalian organs, Physiol. Reviews 59: 527 (1979).Google Scholar
  12. 12.
    W. Griffith and A. Meister, Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine), J. Biol. Chem. 254: 7558 (1979).Google Scholar
  13. 13.
    B. Chance, Spectroscopy of intracellular respiratory pigments, Science 120: 767 (1954).Google Scholar
  14. 14.
    B. Chance and G. Hollunger, The interaction of energy and electron transfer reactions in mitochondria, I, II and III, J. Biol. Chem. 236(2961).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Marie E. Varnes
    • 1
  • John E. Biaglow
    • 1
  • Stephen W. Tuttle
    • 1
  1. 1.Department of Radiology, Division of Biochemical OncologyCase Western Reserve UniversityClevelandUSA

Personalised recommendations