Near Infrared Optical Monitoring of Cat Skeletal Muscle during Hypercapnia

  • N. B. Hampson
  • C. A. Piantadcsi
  • F. F. Jöbsis-VanderVliet
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


Circulatory effects of CO2 have been previously examined in a variety of tissues,1,2,3,4 however, prior efforts to evaluate oxygen transport to skeletal muscle during hypercapnia using non-specific or invasive methods have reported sometimes contrasting conclusions.4,5,6 The recent development of near infrared (NIR), multiple wavelength, differential spectrophotometry has allowed non-invasive assessment of oxygen sufficiency in intact tissues including skeletal muscle7,8 by allowing continuous in situ monitoring of the reduction-oxidation (redox) state of the terminal member of the mitochondrial “respiratory chain”, cytochrome a,a 3. Oxidized cytochrome a,a 3 has an absorption band in the NIR region which is not present when the molecule is reduced. NIR multiple wavelength spectrophotometry allows ongoing assessment of the redox state of a population of cytochrome a,a 3 molecules in a tissue being monitored, with absorption of NIR light increasing as the concentration of oxidized cytochrome a,a 3 increases and absorption decreasing as more molecules in the population become reduced. Furthermore, changes in regional blood volume and saturation can be monitored simultaneously to provide ancillary information useful for interpreting changes in the redox signal. The present study examines the effect of hypercapnia on the steady state of reduction-oxidation of cytochrome a,a 3 and also on the blood volume (t-BV) and hemoglobin plus myoglobin (Hb + Mb and HbO2 + MbO2) saturation responses in intact hindlimb muscles of the cat.


Metabolic Acidosis Indocyanine Green Hypercapnic Acidosis Gallamine Triethiodide Regional Blood Volume 
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  1. 1.
    Lassen, N.A. Cerebral blood flow and oxygen consumption in. man. Physiol. Rev., 39: 183–238 (1959).PubMedGoogle Scholar
  2. 2.
    Lockett, M.F. Effects of changes in PO2, PCO2, and pH on the total vascular resistance of perfused cat kidneys. J. Physiol., London, 193: 671–678 (1967).Google Scholar
  3. 3.
    McGinn, F.P., Mendel, D., and Perry, P.M. The effects of alteration of CO2 and pH on intestinal blood flow in the cat. J. Physiol., London, 192: 669–680 (1967).Google Scholar
  4. 4.
    Kontos, B.A., Richardson, D.W., Patterson, J.L., Jr. Vasodilator effect of hypercapnic acidosis on human forearm blood vessels. Am. J. Physiol., 2.15: 1403–1405 (1968).Google Scholar
  5. 5.
    Whalen, W.J. and Nair, P. Skeletal muscle P02: effect of inhaled and topically applied 02 and CO2. Am. J. Physiol., 218: 973–980 (1970).PubMedGoogle Scholar
  6. 6.
    Radawski, D., Dabney, J.M., Daugherty, R.M. Jr., Haddy, F.J., and Scott, J.A. Local effects of CO2 on vascular resistances and weight of the dog forelimb. Am. J. Physiol., 222: 439–443 (1972).PubMedGoogle Scholar
  7. 7.
    Jöhsis-VanderVliet, F.F. Noninvasive, near Infrared monitoring of cellular oxygen sufficiency in vivo. Adv. Exp. Med. Biol., In press.Google Scholar
  8. 8.
    Piantadosi, C.A. and Jöbsis-VanderVliet, F.F. Near infrared optical monitoring of intact skeletal muscle during hypoxia and hemorrhagic hypotension in cats. Adv. Exp. Med. Biol., In press.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • N. B. Hampson
    • 1
  • C. A. Piantadcsi
    • 1
  • F. F. Jöbsis-VanderVliet
    • 1
  1. 1.Departments of Medicine and PhysiologyDuke University Medical CenterDurhamUSA

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