In Vivo Effects of PAF-Acether and Arterial Thrombosis

  • R. H. Bourgain
  • L. Maes
  • R. Andries
  • P. Braquet
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


Platelet-activating factor (PAF) was first described in 1972 (1), after a soluble intermediate was demonstrated originating from rabbit leukocytes and thought to be responsible for the liberation of histamine from rabbit platelets (2). Structural analysis indicated that PAF was a 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (3); as such it was termed PAF-acether.


Platelet Activate Factor Arterial Thrombosis Rabbit Platelet Platelet Thrombus Arthus Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. Benveniste, C. C. Cochrane and P. M. Henson, Leukocyte dependent histamine release from rabbit platelets: the role of IgE, basophile and a platelet activating factor, J. Exp. Med. 136: 1356 (1972).PubMedCrossRefGoogle Scholar
  2. 2.
    R. P. Siragnian and A. G. Osler, Destruction of rabbit platelets in the allergic response of sensitized leukocytes. I/ Demonstration of a fluid phase intermediate, J. Immunol. 106: 1244 (1971).Google Scholar
  3. 3.
    J. Benveniste, J. P. Le Couedic, J. Polonski and M. Tence, Structural analysis of purified platelet-activating factor by lipase, Nature 269: 170 (1979).CrossRefGoogle Scholar
  4. 4.
    J. Benveniste, Platelet-activating factor (PAF-acether): present status, Agents and Actions 11: 542 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    J. Benveniste, J. Egido and V. Gutierrez-Millet, Evidence for the involvement of the IgE-basophil system in acute serum sickness of rabbits, Clin. Exp. Immunol. 26: 449 (1976).PubMedGoogle Scholar
  6. 6.
    T. C. Kravis and “P. M. Henson, Accumulation of platelets at the site of antigen-antibody mediated injury: a possible role for IgE antibody and mast cells, J. Immunol. 188: 1569 (1977).Google Scholar
  7. 7.
    L. M. McManus, J. Hanahan, C. A. Demopoulos and R. M. Pinckard, Pathobiology of the intravenous infusion of acetyl-glyceryl ether phosphocholine (AGEPC), a synthetic platelet activating factor (PAF) in the rabbit, J. Immunol. 124: 2919 (1980).PubMedGoogle Scholar
  8. 8.
    B. B. Vargaftig, H. Lefort, M. Chignard and J. Benveniste, Platelet-activating factor induces a platelet dependent bronchoconstriction unrelated to the formation of prostaglandin derivatives, Eur. J. Pharmac. 65: 185 (1980).CrossRefGoogle Scholar
  9. 9.
    J. P. Cazenave, J. Benveniste and J. F. Mustard, Aggregation of rabbit platelets by platelet activating factor is dependent of the release reaction and arachidonate pathway and inhibited by membrane active drugs, Lab. Invest. 41: 275 (1979).PubMedGoogle Scholar
  10. 10.
    M. Chignard, J. P. Le Couedic, M. Tence, B. B. Vargaftig and J. Benveniste, The role of platelet-activating factor in platelet aggregation, Nature 179: 799 (1979).CrossRefGoogle Scholar
  11. 11.
    D. H. Nam and J. A. High, Thrombin-induced formation of a polar lipid material within platelets that possess platelet-activating activity, Thromb. Res. 20: 285 (1980).CrossRefGoogle Scholar
  12. 12.
    G. Camussi, M. Aglietta, F. Malavazi, C. Tetta, W. Piacibello, F. Sanavio and F. Bussolino, The release of platelet-activating factor from human endothelial cells in culture, J. Immunol. 131: 2397 (1983).PubMedGoogle Scholar
  13. 13.
    S. Prescott, G. Zimmerman and T. McIntyre, Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-snglycero-3-phosphocholine) when stimulated with thrombin, Proc. Natl. Acad. Sci. USA 81: 3534 (1984).PubMedCrossRefGoogle Scholar
  14. 14.
    M. Chignard, J. P. Le Couedic, B. B. Vargaftig and J. Benveniste, Platelet-activating factor (PAF-acether) secretion from platelets: effects of aggregating agents, Brit. J. Haematol. 46: 455 (1980).CrossRefGoogle Scholar
  15. 15.
    J. T. O’Flaherty, C. M. Miller, J. C. Lewis, R. L. Wykle, D. A. Bass, C. E. McCall, M. Waite and L. R. De Chatelet, Neutrophyl responses to platelet-activating factor, Inflammation 5 (3): 193 (1981).PubMedCrossRefGoogle Scholar
  16. 16.
    P. Braquet, R. F. Vidal, M. Braquet, H. Hamard and B. B. Vargaftig, Involvement of leukotrienes and PAF-acether in the increased micro-vascular permeability of the rabbit retina, Agents and Actions 15: 82 (1984).CrossRefGoogle Scholar
  17. 17.
    M. Sanchez-Crespo, P. Inarrea, V. Alvarez, F. Alonzo, J. Egido and L. Hernando, Presence in normal urine of a hypotensive and platelet-activating phospholipid, Am. J. Physiol. 244: F706 (1983).PubMedGoogle Scholar
  18. 18.
    P. Bessin, J. Bonnet, D. Apffel, C. Soulard, L. Dessroux, I. Pelas and J. Benveniste, Acute circulatory collapse caused by platelet-activating factor (PAF-acether) in dogs, Eur. J. Pharmacol. 86: 403 (1983).PubMedCrossRefGoogle Scholar
  19. 19.
    Y. Kasuya, Y. Masuda and K. Shigenobu, The possible role of endothelium in the vasodilation response of rat thoracic aorta to platelet activating factor (PAF), J. Pharmacobiod. 7: 138 (1984).CrossRefGoogle Scholar
  20. 20.
    R. H. Bourgain and F. Six, A continuous registration method in experimental arterial thrombosis in the rat, Thromb. Res. 4: 599 (1974).PubMedCrossRefGoogle Scholar
  21. 21.
    R. H. Bourgain, H. Vermariën, R. Andries, F. Vereecke, J. Jacqueloot, J. Rennies, E. Blockeel and F. Six, A standardized ‘in vivo’ model for the study of experimental arterial thrombosis: description of a method, in: “Oxygen transport to tissue VI”, D. Bruley, H. I. Bicher and D. Reneau, eds., Plenum, New York (1985).Google Scholar
  22. 22.
    M. A. Reidy and S. M. Schwartz, A technique to investigate morphology and endothelial cell replication of small arteries: a study in acute angiotensine-induced hypertensive rats, Microvasc. R. 24: 188 (1982).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • R. H. Bourgain
    • 1
  • L. Maes
    • 1
  • R. Andries
    • 1
  • P. Braquet
    • 2
  1. 1.Dept. PhysiologyVUBBrusselsBelgium
  2. 2.Institut Henri BeaufourLe Plessis RobinsonFrance

Personalised recommendations