Advertisement

Kinetic Analysis of Cardiac β-Receptors in Perfused Working Rabbit Hearts

  • H. M. Rhee
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)

Summary

Myocardial adrenergic β-receptors were isolated and partially purified from the nonischemic perfused working rabbit hearts. Using highly radioactive β-receptor antagonist, dihydroalprenolol (DHA), properties of β-receptors were investigated by kinetic equilibrium analysis when the physiological function of the heart appeared to be normal. At the concentration of 10 nM DHA dissociation constant (Kd) was 14.9 nM and there were at least two distinctly different DHA binding sites, based on the analysis of the dissociation rate of DHA-receptor complex. Identification of the two distinctly different DHA binding sites was not obvious from the analysis of Scatchard plot.

Keywords

Dissociation Rate Rabbit Heart Scatchard Plot Scatchard Analysis Rapid Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Changeux, J. P., Kasai, M., and Lee, C. Y. (1970) Proc. Nat. Acad. Sci. USA 67, 1421–1427.CrossRefGoogle Scholar
  2. 2.
    Lefkowitz, R. J., Mukherjee, C., Coverstone, M., and Caron, M. C. (1974) Biochem. Biophys. Res. Comm. 60 (2), 703–709.CrossRefGoogle Scholar
  3. 3.
    Winek, R., and Bhalla, R. (1979) Biochem. Biophys. Res. Comm. 91 (1), 200–206.CrossRefGoogle Scholar
  4. 4.
    Hoffman, B. B., and Lefkowitz, R. J. (1982) Ann. Rev. Physiol. 44, 475–484.CrossRefGoogle Scholar
  5. 5.
    Martin, W. R. (1983) Pharmacol. Rev. 35, 283–323.PubMedGoogle Scholar
  6. 6.
    Freychet, P., Roth, J., and Neville, D. M., Jr. (1971) Biochem. Biophys. Res. Comm. 43, 400–408.PubMedCrossRefGoogle Scholar
  7. 7.
    Mukherjee, C., Caron, M. G., and Lefkowitz, R. J. (1975) Proc. Nat. Acad. Sci. USA 72, 1945–1949.PubMedCrossRefGoogle Scholar
  8. 8.
    Mukherjee, H., Wong, T. M., Buja, L. M., and Lefkowitz, R. J. (1979) J. Clin. Invest. 64, 1423–1428.CrossRefGoogle Scholar
  9. 9.
    Corr, P. B., Shayman, J. A., Kramer, J. B., and Kipnis, R. J. (1981) J. Clin. Invest. 67, 1232–1236.CrossRefGoogle Scholar
  10. 10.
    Woodcock, E. A., Funder, J. W., and Johnson, C. I. (1979) Circ. Res. 45 (4), 560–565.PubMedGoogle Scholar
  11. 11.
    Bertel, 0., Bühler, F. R., Kiowski, W., and Liitold, B. E. (1979) Hypertension 2 (2), 130–138.Google Scholar
  12. 12.
    Limas, C. J., and Limas, C. (1984) Circ. Res. 55 (4), 524–531.PubMedGoogle Scholar
  13. 13.
    Harden, T. K., Mailman, R. B., Mueller, R. A., and Breese, G. R. (1979) Brain Res. 166, 194–198.PubMedCrossRefGoogle Scholar
  14. 14.
    Fraser, C. M., Venter, J. C., and Kaliner, M. (1981) New Eng. J. Med. 305, 1165–1170.PubMedCrossRefGoogle Scholar
  15. 15.
    Rhee, H. M., and Tyler, L. (1984) International Society of Oxygen Transport to Tissue, 1984 Meeting in Nijmegen, Netherland.Google Scholar
  16. 16.
    Rhee, H. M., and Cooper, J. (1984) Oxygen Transport to Tissue, Vol. V, Eds. D. W. Lubbers et al., Plenum Publishing Corp., pp. 389–402.Google Scholar
  17. 17.
    Alexander, R. W., Williams, L. T., and Lefkowitz, R. J. (1975) Proc. Nat. Acad. Sci. 72 (4), 1564–1568.CrossRefGoogle Scholar
  18. 18.
    Scatchard, G. (1949) Ann. N. Y. Acad. Sci. 51, 660–672.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • H. M. Rhee
    • 1
  1. 1.Department of PharmacologyOral Roberts University School of MedicineTulsaUSA

Personalised recommendations