A Micro-System for Measuring Oxygen Kinetic Parameters of Biopterin Linked Hydroxylations: L-Dopa Synthesis and Parkinson’s Disease

  • B. Hagihara
  • G. Devathasan
  • M. McCabe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


Because of difficulties in the purification and assay of the hydroxylating system for the synthesis of L-dopa, little is known of the detailed (oxygen) kinetics of the enzymes involved in this reaction in the brain. However, related liver enzymes have been purified to homogeneity, and kinetic studies have revealed a considerable complexity, where substrate oxidation is dependent upon the presence of phospholipids in particular. Several such additive co-factors can alter the rate versus substrate plot from a sigmoidal curve to one which is hyperbolic.


Tyrosine Hydroxylase Oxygen Partial Pressure Fractional Oxygen Extraction Electrode Output Combine Electrode 


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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • B. Hagihara
    • 1
  • G. Devathasan
    • 2
  • M. McCabe
    • 2
  1. 1.Department of BiochemistryOsaka University MedicalOsaka 530Japan
  2. 2.Department of MedicineNational University of SingaporeKent RidgeSingapore

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