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A New Method for Measuring Oxygen Concentration in Biological Systems

  • Jane M. Vanderkooi
  • David F. Wilson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)

Abstract

Accurate and rapid measurement of the oxygen concentration in biological samples, particularly when these oxygen concentrations are low, has been the goal of many research workers. By far the most successful method has been the use of oxygen electrodes (for review see Silver, 1984). However it is limited by the stability of the electrode surface and by instabilities in the oxygen diffusion barrier (because it measures the rate of diffusion of oxygen to the cathode). An alternate method, which measures the oxygen dependent quenching of the fluorescence of pyrene butyric acid (Vaughan and Weber, 1970; Knopp and Longmuir, 1972; Opitz and Lübbers, 1984), has been much less useful because the fluorescence intensity is affected by many experimental parameters other than oxygen concentration.

Keywords

Oxygen Concentration Triplet State Excited Singlet State Excited Triplet State Dimethyl Ester 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Horie, T. and Vanderkooi, J.M. (1984) Use of phosphorescence at room temperature for the study of biological molecules, Life Chemistry Reports 2, 141–178Google Scholar
  2. Knopp, J.A. and Longmuir, I.S. (1972) Intracellular measurement of oxygen by quenching of fluorescence of pyrene butyric acid, Biochim. Biophys. Acta 279, 393–397.Google Scholar
  3. Opitz, N. and Lubbers, D.W. (1984) Increased resolution power in PO analysis at lower PO„ levels via sensitivity enhanced optical Po sensors (PO optodes) using fluorescence dyes. Adv. in Exptl. Mes. Biot. 180, 2261–267.Google Scholar
  4. Silver, I.A. (1984) Polarographic techniques of oxygen measurements in “Oxygen: An in depth study of its pathophysiology” (S.F. Gottlieb, I.S. Longmuir and J.R. Totter, eds.) Undersea Med. Soc. pub No. 62(ws) 3–1–84 p. 215–238.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Jane M. Vanderkooi
    • 1
  • David F. Wilson
    • 1
  1. 1.Department of Biochemistry and Biophysics, School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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