The Solubility of Oxygen in Erythrocyte Ghosts and the Flux of Oxygen Across the Red Cell Membrane

  • M. McCabe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


A composite of partition and diffusion coefficients, together with tissue architecture, controls the movement of oxygen gas within tissues. One aspect of tissue architecture concerns the cell membrane and its role as a possible limiting factor in the flux of oxygen. In general the flux of a solute across a membrane is expressed as a permeability coefficient (due to uncertainties of membrane thickness). The relationship between permeability and diffusion has been discussed by Leib & Stein (1971).


Electron Spin Resonance Partition Coefficient Metal Plate Membrane Phase Tritiated Water 
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  1. Backer, J.M., Budker, V.G., Eremenko, S.I. and Mo1in, Y.N., 1977. Detection of the kinetics of biochemical reactions with oxygen using exchange broadening in the ESR spectra of nitroxide radicles. Biochem. Biophys. Acta 460, 152.Google Scholar
  2. Black, M., 1962. The permeability of monolayers to several gases, in “Retardation of Evaporation Monolayers”. V. K. La Mer, ed., Academic Press, New York.Google Scholar
  3. Carlson, E. and Comroe, J.H., 1958. The rate of uptake of carbon monoxide and of nitric oxide by normal human erythrocytes and by experimentally produced spherocytes. J. Gen. Physiol. 42, 83.Google Scholar
  4. Crosby, W.H., Munn, J.I. and Furth, F.W., 1954. Standardizing a method for clinical hemoglobinometry. U.S. Armed Forces Med. J. 5, 693.Google Scholar
  5. Dodge, J.T., Mitchell, C. and Hanahan, D.J., 1963. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys. 100, 119.Google Scholar
  6. Elrick, D.E., Smiles, D. and Woodgin, R.A., 1972. Double membrane diaphragm techique for absolute measurements of diffusion coefficients. J. Chem. Soc. Faraday Trans. 68, 591.Google Scholar
  7. Fischkoff, S. and Vanderkooi, J.M., 1975. Oxygen diffusion in biological and artificial membranes determined by the fluorochrome pyrene. J. Gen. Physiol. 65, 663.Google Scholar
  8. Ginzburg, B.Z. and Katchaisky, A., 1963. Frictional coefficients of the flows of non electrolytes through artificial membranes. J. Gen. Physiol. 47, 403 (-18).Google Scholar
  9. Hagihara, B., Devathasan, G. and McCabe, M., 1985. A microsystem for measuring oxygen kinetic parameters: biopterin linked hydroxylations and L-dopa synthesis in “Oxygen Transport to Tissues, VIII” p, ed. I. S. Longmuir, Plenus Press, New York.Google Scholar
  10. Knopp, J.A. and Longmuir, I.S., 1972. Intracellular measurement of oxygen by quenching of fluorescence of pyrenebutyric acid. Biochim. Biophys. Acta 279, 393.Google Scholar
  11. Kreuzer, F. and Yahr, W.Z., 1960. Influence of red cell membrane on diffusion of oxygen. J. Appl. Physiol. 15, 1117.Google Scholar
  12. Leib, W.R. and Stein, W.D., 1971. Implications of two different types of diffusion in biological membranes. Nature (New Biology) 234, 220.Google Scholar
  13. McCabe, M., 1967 (a and b). Diffusion coefficients in polymer solutons. Biochem. J. 104, 8p, 9 p.Google Scholar
  14. McCabe, M. and Laurent, T., 1975. Diffusion of oxygen, nitrogen and water in hyaluronate solutions. Biochem. Biophys. Acta 399, 131.Google Scholar
  15. Povich, M.J., 1975. Measurement of dissolved oxygen concentration and diffusion coefficients by electron spin resonance. J. Phys. Chem. 79, 106.Google Scholar
  16. Roughton, F.J.W., 1959. Diffusion and simultaneous chemical reaction velocity in hemoglobin solutions and red cell suspensions. In “Progress in Biophysics and Biophysical Chemistry” 9, 55, ed Butler, J.A.V. and Katz, B. Pergamon Press, London.Google Scholar
  17. Shaafi, R.J., Gary-Bobo, C.M. and Solomon, A.K., 1971. Permeability of red cell membranes to small hydrophilic and lipophilic solutes. J. Gen. Physiol. 58, 238.Google Scholar
  18. Windrem, D.A. and Plachy, W.Z., 1980. The diffusion-solubility of oxygen in lipid bilayers. Biochim. Biophys. Acta 600, 655.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • M. McCabe
    • 1
  1. 1.James Cook UniversityAustralia

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