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O2 Optodes for Analyzing Micro Blood Samples Using Thin Sensor Layers with Small O2 Capacities and Special Reflection Properties for Optical Decoupling of Sensor and Sample

  • Norbert Opitz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)

Abstract

By incorporating an O2-sensitive fluorescence indicator into a poly­meric membrane the membrane can be used to measure the pO2 in a sample (Lübbers and Opitz, 1983) or on organ surfaces (Opitz and Lübbers, this volume). However, with measurements in different samples, e.g. blood and gas, reflection changes occur at the optical phase transition sensor/ medium due to different optical densities of the various media to be measured. These reflection changes originating from both excitation- and fluorescence radiation (impinging either directed or undirected on the phase transition, respectively), cannot be eliminated by a simple refe­rence measurement with the aid of a fluoresence standard, because of the wavelength dependence of the refractive index n (optical dispersion). As an example, Fig. 1 shows two corrected excitation spectra (λem = 420 nm) of an O2-sensitive perylene derivative (Kroneis and Marsoner, 1983) monitored by means of an optode-device for micro blood samples (sample volume ca. 70/ul) in gas and Ringer’s solution, both equilibrated with 20 % oxygen. Since both spectra differ significantly, calibration curves in various media are not identical.

Keywords

Fluorescence Emission Excitation Radiation Reflection Change Oxygen Capacity Direct Fluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Knopp, J.A., and Longmuir, I.S., 1972, Intracellular measurement of oxygen by quenching of fluorescence of pyrenebutyric acid. Biochim. Biophys. Acta, 279: 393–397.Google Scholar
  2. Kroneis, H.W., and Marsoner, H.J., 1983, A fluorescence-based sterilizable oxygen probe for use in bioreactors. Sensors and Actuators, 4: 587–592.CrossRefGoogle Scholar
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  4. Opitz, N., and Lubbers, D.W. (this volume), Oxygen pressure measurements on moving organ surfaces by fluorescence sensor membranes using contactles signal transmission via fluorescence sensor radiation.Google Scholar
  5. Vaughan, W.M., and Weber, G., 1970, Oxygen quenching of pyrenebutyric acid fluorescence in water. A dynamic probe of the microenvironment. Biochem. 9: 464.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Norbert Opitz
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmundGermany

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