Improved Quantitative Analysis of Reflection Spectra Obtained from the Surface of the Isolated Perfused Guinea Pig Heart

  • J. Hoffmann
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


The chromophoric substances which cause light absorption of optical spectra of the perfused Langendorff heart in the wavelength range of 500 to 650 nm are myoglobin and the cytochromes of the respiratory chain. We use a modified multicomponent analysis based on the two-flux theory of Kubelka and Munk (1931; Kubelka, 1948; 1954) to quantify the reflection spectra. Since the absorption spectra of the involved substances have overlapping absorption peaks and different forms, their concentration ratios are determined within different accuracy. The experiments show that the accuracy can be improved by the introduction of constraints, e.g. the reduction of the number of the basic absorption spectra. The constraints are worked out for the special measuring conditions.


Reflection Spectrum Basic Spectrum Multicomponent Analysis Perfuse Langendorff Heart Give Correlation Coefficient 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • J. Hoffmann
    • 1
  • D. W. Lübbers
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmund 1Germany

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