Estimation of Concentration Ratios and the Redox States of the Cytochromes from Noisy Reflection Spectra Using Multicomponent Analysis Methods
To quantify tissue reflection spectra, multicomponent analysis methods have been successfully used. These methods reconstruct the measured reflection spectra using a set of basic absorption spectra, which describe the single chromophoric substances of the multicomponent system, and applying the Kubelka-Munk algorithm (Kubelka and Munk, 1931; Hoffmann et al., 1984). Since basic absorption spectra differ in distinctness and sharpness of absorption peaks, multicomponent analysis methods give concentration ratios of the components with different uncertainties.
KeywordsRedox State Concentration Ratio Reflection Spectrum Error Weight Basic Spectrum
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