Estimation of Concentration Ratios and the Redox States of the Cytochromes from Noisy Reflection Spectra Using Multicomponent Analysis Methods

  • J. Hoffmann
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


To quantify tissue reflection spectra, multicomponent analysis methods have been successfully used. These methods reconstruct the measured reflection spectra using a set of basic absorption spectra, which describe the single chromophoric substances of the multicomponent system, and applying the Kubelka-Munk algorithm (Kubelka and Munk, 1931; Hoffmann et al., 1984). Since basic absorption spectra differ in distinctness and sharpness of absorption peaks, multicomponent analysis methods give concentration ratios of the components with different uncertainties.


Redox State Concentration Ratio Reflection Spectrum Error Weight Basic Spectrum 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • J. Hoffmann
    • 1
  • D. W. Lübbers
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmund 1Germany

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