Genetic Analyses of the Roles of UmuDC and MucAB in Mutagenesis
Mutagenesis by some alkylating agents and base analogs in Escherichia coli appears to involve a simple mispairing during replication. In contrast, mutagenesis by ultraviolet (UV) light and many chemicals in E. coli requires the participation of an inducible process that is dependent on the products of the umuD, umuC, and recA genes. The requirement for induced cellular functions for UV mutagenesis was first noted by Weigle (37), who found that UV was not mutagenic for bacteriophage lambda unless the bacteria had been preirradiated with UV. The induction of this process was later shown by Defais et al. (7) to be blocked by recA(Def) or lexA(Ind) mutations. The fact that the same recA(Def) and lexA(Ind) mutations rendered E. coli nonmutable by UV and many chemicals (16,24,39) indicated that mutageneses of the bacterial chromosome required the participation of the same recA + lexA + -controlled process, and the inducibility of the system was demonstrated by subsequent physiological experiments (40).
KeywordsRecA Protein Chemical Mutagenesis MucA Protein LexA Protein LexA Binding
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