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Nucleotide Excision Repair Genes from the Yeast Saccharomyces Cerevisiae

  • Errol C. Friedberg
  • Reinhard Fleer
  • Louie Naumovski
  • Charles M. Nicolet
  • Gordon W. Robinson
  • William A. Weiss
  • Elizabeth Yang
Part of the Basic Life Sciences book series (BLSC, volume 39)

Abstract

The genetics of nucleotide excision repair in the yeast Saccharomyces cerevisiae is complex, apparently requiring at least 10 genes. We have isolated 5 of these genes (designated RAD1, RAD2, RAD3, RAD4, and RAD1O) by molecular cloning and plan to overexpress them in order to generate proteins for biochemical study. We have sequenced four of these five genes and have noted regions of homology with other proteins in the predicted amino acid sequence of some of them. In particular, there is striking homology between Rad3 protein and a number of prokaryotic and eukaryotic proteins that bind nucleotides and hydrolyze ATP or GTP. Mutations in this region of the RAD3 gene render cells defective in the nucleotide excision repair function. In addition to its role in nucleotide excision repair, the RAD3 gene is essential for the viability of haploid cells in the absence of DNA damage. The nature of the essential function is unknown. The RAD1 and RAD3 genes are not inducible by DNA damaging agents. However, exposure of cells to UV radiation, 4-nitroquinoline 1-oxide, or γ radiation results in 4- to 6-fold enhanced expression of the RAD2 gene.

Keywords

Excision Repair Nucleotide Excision Repair Amino Acid Sequence Homology Nucleotide Excision Repair Gene Excision Repair Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Errol C. Friedberg
    • 1
  • Reinhard Fleer
    • 1
  • Louie Naumovski
    • 1
  • Charles M. Nicolet
    • 1
  • Gordon W. Robinson
    • 1
  • William A. Weiss
    • 1
  • Elizabeth Yang
    • 1
  1. 1.Department of PathologyStanford University School of MedicineStanfordUSA

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