A Better Way to Make RNA for Physical Studies
Studies on the structure and function of RNA have often been hampered by the difficulty in obtaining material in sufficient amounts for many physical methods. In addition, the variety of RNA sequences easily available for study is somewhat limited. Ideally, one would like a simple method to rapidly obtain multimilligram amounts of any RNA sequence desired. Although methods for the chemical synthesis of RNA have progressed rapidly in the past several years (1), they remain technically quite complex and are generally limited to fragments of less than 20 nucleotides. The enzymatic methods used by this laboratory to synthesize RNA by joining shorter fragments with T4 RNA ligase have also succeeded in preparing fragments up to 20 nucleotides long, but yield only very small amounts of material (2).
KeywordsTemplate Strand Type Template Runoff Transcription Runoff Transcript Yeast tRNAPhe
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