Advertisement

Z-RNA: A Left-Handed Double Helix

  • Ignacio TinocoJr.
  • Phillip Cruz
  • Peter Davis
  • Kathleen Hall
  • Charles C. Hardin
  • Richard A. Mathies
  • Joseph D. Puglisi
  • Mark A. Trulson
  • W. Curtis JohnsonJr.
  • Thomas Neilson
Part of the NATO ASI Series book series (NSSA, volume 110)

Abstract

The synthetic polyribonucleotide, poly [r(G-C)], has been shown to undergo a transition from the right handed A-form to a left-handed Z-conformation.1 This is the first major conformational change found in double-stranded RNA.2 Therefore it is important to corroborate this observation, to characterize the conditions that produce Z-RNA, and to determine some of the properties of this novel left-handed structure. This article is a summary of our results in this area.

Keywords

Circular Dichroism Circular Dichroism Spectrum Single Strand Circular Dichroism Measurement Slow Exchange 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    K. Hall, P. Cruz, I. Tinoco, Jr., T. M. Jovin, and J. H., van de Sande, Nature 311, 584–586 (1984).CrossRefGoogle Scholar
  2. 2.
    W. Saenger, Principles of Nucleic Acid Structure, Springer-Verlag, New York, Chapter 9, 1984.CrossRefGoogle Scholar
  3. 3.
    K. Hall, P. Cruz, and M. J. Chamberlin, Arch. Biochem. Biophys. 236, 47–51 (1985).CrossRefGoogle Scholar
  4. 4.
    D. J. Patel, S. A. Kozlowski, A. Nordheim and A. Rich, Proc. Natl. Acad. Sci., U. S. A. 79, 1413–1417 (1982).CrossRefGoogle Scholar
  5. 5.
    T. M. Jovin, J. H. van de Sande, D. A. Zarling, D. J. Arndt-Jovin, F. Eckstein, H. H. Fuldner, C. Greider, I. Grieger, R. Hamori, B. Kalisch, L. P. Mclntosh and M. Robert-Nicoud and L. B. Clark, Cold Spring Harbor Symp. Quant Biol. 47, 143–154 (1983).CrossRefGoogle Scholar
  6. 6.
    J. H. Riazance, W. A. Baase, W. C. Johnson, Jr., K. Hall, P. Cruz, I. Tinoco, Jr., Nucleic Acids Res., 13, 4983–4989 (1985).CrossRefGoogle Scholar
  7. 7.
    A. Williams, Jr., C. Cheong, I. Tinoco, Jr., unpublished results.Google Scholar
  8. 8.
    I. Tinoco, Jr., C. Bustamante and M. F. Maestre, Ann. Rev. Biophys. Bioeng. 9, 107–141 (1980).CrossRefGoogle Scholar
  9. 9.
    V. Rizzo and J. A. Schellman, Biopolymers, 23, 435–470 (1984).CrossRefGoogle Scholar
  10. 10.
    L. B. Clark, J. Am. Chem. Soc. 99, 3934–3938 (1977).CrossRefGoogle Scholar
  11. 11.
    T. J. Thamann, R. C. Lord, A. H.-J. Wang and A. Rich, Nucleic Acids Res. 9, 5443–5457 (1981).CrossRefGoogle Scholar
  12. 12.
    M. Behe and G. Felsenfeld, Proc. Natl. Acad. Sci., U. S. A. 78, 1619–1623 (1981).CrossRefGoogle Scholar
  13. 13.
    R. V. Gessner, G. J. Quigley, A. H.-J. Wang, G. A. van de Marel, J. H. van Boom and A. Rich, Biochemistry 24, 237–240 (1985).CrossRefGoogle Scholar
  14. 14.
    A. Moller, A. Nordheim, S. A. Kozlowski, D. J. Patel and A. Rich, Biochemistry 23, 54–62 (1984).CrossRefGoogle Scholar
  15. 15.
    B. G. Feurstein, L. J. Marton, M. A. Keniry, D. L. Wade and R. H. Shafer, Nucleic Acids Res. 13, 4133–4141 (1985).CrossRefGoogle Scholar
  16. 16.
    K. Hall, Ph.D. Thesis, U. of California, 1984.Google Scholar
  17. 17.
    H. F. Noller and J. A. Lake, Chapter 5 in Membrane Structure and Function, Vol. 6, E. E. Bittar, editor, John Wiley, New York, 1984.Google Scholar
  18. 18.
    H. F. Noller, Ann. Rev. Biochem. 53, 119–162 (1984).CrossRefGoogle Scholar
  19. 19.
    R. R. Guttell, personal communication.Google Scholar
  20. 2O.
    C. R. Woese, R. R. Guttell, R. Gupta and H. F. Noller, Microbiol. Revs. 47, 621–669 (1983).Google Scholar
  21. 21.
    M. J. Leibowitz, D. J. Thiele and E. M. Hannig, pp. 457–462 in Double Stranded RNA Viruses, R. W. Compan and D. H. L. Bishop, editors, Elsevier Science, Amsterdam, 1983.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Ignacio TinocoJr.
    • 1
  • Phillip Cruz
    • 1
  • Peter Davis
    • 1
  • Kathleen Hall
    • 1
  • Charles C. Hardin
    • 1
  • Richard A. Mathies
    • 1
  • Joseph D. Puglisi
    • 1
  • Mark A. Trulson
    • 1
  • W. Curtis JohnsonJr.
    • 2
  • Thomas Neilson
    • 3
  1. 1.Department of Chemistry and Laboratory of Chemical BiodynamicsUniversity of CaliforniaBerkeleyUSA
  2. 2.Department of Biochemistry and BiophysicsOregon State UniversityCorvallisUSA
  3. 3.Department of BiochemistryMcMaster UniversityHamiltonCanada

Personalised recommendations