Mutagenesis of Ribosomal RNA as a Method to Investigate Interactions Between rRNA, mRNA and tRNA
Our understanding of the three dimensional structure of ribosomal RNA is just beginning to unfold. Information has come from a variety of approaches including crosslinking experiments of rRNA (1–3) and neutron and proton magnetic resonance studies (4). Comparative phylogenetic studies, so useful for determining the secondary structure of ribosomal RNA (5), may now also be applied to the study of tertiary structure (Noller, personal communication and 6). Another new approach to the study of rRNA structure involves the application of genetic techniques to introduce mutations in the rRNA (7). This latter approach, while in its infancy, provides the means to confirm results obtained by other methods, as well as explore new areas.
KeywordsMutant Plasmid Multicopy Plasmid Suppressor tRNA rRNA Structure Mutant Ribosome
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